Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.

Linnéa Taylor; Karin Arnér; Karl Engelsberg; Fredrik Ghosh

doi:10.1016/j.ijdevneu.2015.03.002

Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.

Linnéa Taylor, Karin Arnér, Karl Engelsberg, Fredrik Ghosh

Ophthalmology, Lund

Research output: Contribution to journal › Article › peer-review

Abstract

PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development.
MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry.
RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner.
CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.

Original language	English
Pages (from-to)	46-58
Journal	International Journal of Developmental Neuroscience
Volume	42
DOIs	https://doi.org/10.1016/j.ijdevneu.2015.03.002
Publication status	Published - 2015

Subject classification (UKÄ)

Ophthalmology

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10.1016/j.ijdevneu.2015.03.002

http://www.ncbi.nlm.nih.gov/pubmed/25758423?dopt=Abstract

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title = "Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.",

abstract = "PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development. MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry. RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner. CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.",

author = "Linn{\'e}a Taylor and Karin Arn{\'e}r and Karl Engelsberg and Fredrik Ghosh",

year = "2015",

doi = "10.1016/j.ijdevneu.2015.03.002",

language = "English",

volume = "42",

pages = "46--58",

journal = "International Journal of Developmental Neuroscience",

issn = "1873-474X",

publisher = "Elsevier",

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TY - JOUR

T1 - Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.

AU - Taylor, Linnéa

AU - Arnér, Karin

AU - Engelsberg, Karl

AU - Ghosh, Fredrik

PY - 2015

Y1 - 2015

N2 - PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development. MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry. RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner. CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.

AB - PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development. MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry. RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner. CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.

U2 - 10.1016/j.ijdevneu.2015.03.002

DO - 10.1016/j.ijdevneu.2015.03.002

M3 - Article

C2 - 25758423

SN - 1873-474X

VL - 42

SP - 46

EP - 58

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

ER -

Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.

Abstract

Subject classification (UKÄ)

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