Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Svetlana Sapelnikova; Eva Dock; R Solna; P Skladal; Tautgirdas Ruzgas; Jenny Emnéus

doi:10.1007/s00216-003-2000-4

Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Svetlana Sapelnikova, Eva Dock, R Solna, P Skladal, Tautgirdas Ruzgas, Jenny Emnéus

Centre for Analysis and Synthesis

Research output: Contribution to journal › Article › peer-review

Abstract

Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

Original language	English
Pages (from-to)	1098-1103
Journal	Analytical and Bioanalytical Chemistry
Volume	376
Issue number	7
DOIs	https://doi.org/10.1007/s00216-003-2000-4
Publication status	Published - 2003

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

Analytical Chemistry

Keywords

wastewater characterisation
tyrosinase and
horseradish peroxidase-modified electrode
enzyme electrode
screen-printed multienzyme array
flow-injection analysis
amperometric batch and flow system
cholinesterase-modified electrode

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10.1007/s00216-003-2000-4

Cite this

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title = "Screen-printed multienzyme arrays for use in amperometric batch and flow systems",

abstract = "Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.",

keywords = "wastewater characterisation, tyrosinase and, horseradish peroxidase-modified electrode, enzyme electrode, screen-printed multienzyme array, flow-injection analysis, amperometric batch and flow system, cholinesterase-modified electrode",

author = "Svetlana Sapelnikova and Eva Dock and R Solna and P Skladal and Tautgirdas Ruzgas and Jenny Emn{\'e}us",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)",

year = "2003",

doi = "10.1007/s00216-003-2000-4",

language = "English",

volume = "376",

pages = "1098--1103",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer",

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TY - JOUR

T1 - Screen-printed multienzyme arrays for use in amperometric batch and flow systems

AU - Sapelnikova, Svetlana

AU - Dock, Eva

AU - Solna, R

AU - Skladal, P

AU - Ruzgas, Tautgirdas

AU - Emnéus, Jenny

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2003

Y1 - 2003

N2 - Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

AB - Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

KW - wastewater characterisation

KW - tyrosinase and

KW - horseradish peroxidase-modified electrode

KW - enzyme electrode

KW - screen-printed multienzyme array

KW - flow-injection analysis

KW - amperometric batch and flow system

KW - cholinesterase-modified electrode

U2 - 10.1007/s00216-003-2000-4

DO - 10.1007/s00216-003-2000-4

M3 - Article

C2 - 12856095

SN - 1618-2642

VL - 376

SP - 1098

EP - 1103

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 7

ER -

Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Abstract

Bibliographical note

Subject classification (UKÄ)

Keywords

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Cite this