Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

Alejandra Martinez, Osvaldo Delgado, M D Baigori, F Sineriz

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)

Abstract

The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).
Original languageEnglish
Pages (from-to)545-550
JournalBiotechnology Letters
Volume27
Issue number8
DOIs
Publication statusPublished - 2005

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • purification
  • Bacillus halodurans MIR32
  • over-expression
  • sequence
  • xylanase

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