Short leucine-rich glycoproteins of the extracellular matrix display diverse patterns of complement interaction and activation.

Andreas Holmér, Gavin Manderson, Matthias Mörgelin, Anthony J Day, Dick Heinegård, Anna Blom

Research output: Contribution to journalArticlepeer-review

98 Citations (SciVal)

Abstract

The extracellular matrix consists of structural macromolecules and other proteins with regulatory functions. An important family of the latter class of molecules found in most tissues is the small leucine-rich repeat proteins (SLRPs). We have previously shown that the SLRP fibromodulin binds directly to C1q and activates the classical pathway of complement. In the present study we further examine the interactions between SLRPs and complement. Osteoadherin, like fibromodulin, binds C1q and activates the classical pathway strongly while moderate activation is seen in the terminal pathway. This can be explained by the interaction of fibromodulin and osteoadherin with factor H, a major soluble inhibitor of complement. Also, chondroadherin was found to bind C1q and activate complement, albeit to a lesser extent. Chondroadherin also binds factor H. We confirm published data showing that biglycan and decorin bind C1q but do not activate complement. In this study a similar pattern is seen for lumican although its affinity for C1q is lower than for biglycan and decorin. Furthermore, using electron microscopy and radiolabeled SLRPs, we demonstrate two different classes of SLRP binding sites on C1q, to head and stalk respectively, where only binding to the head appears to be activating. We propose a role for SLRPs in the regulation of complement activation in diseases involving the extracellular matrix, particularly those characterized by chronic inflammation such as rheumatoid arthritis, atherosclerosis, osteoarthritis and chronic obstructive lung disease.
Original languageEnglish
Pages (from-to)830-839
JournalMolecular Immunology
Volume46
DOIs
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Division of Infection Medicine (BMC) (013024020), Protein Chemistry (013017510), Connective Tissue Biology (013230151), Clinical Chemistry, Malmö (013016000)

Subject classification (UKÄ)

  • Immunology in the medical area

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