Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate

Jonatan Andersson, Bo Mattiasson

Research output: Contribution to journalArticlepeer-review

63 Citations (SciVal)

Abstract

Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process.
Original languageEnglish
Pages (from-to)88-95
JournalJournal of Chromatography A
Volume1107
Issue number1-2
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • cation exchange
  • whey
  • WPC
  • lactoferrin
  • lactoperoxidase
  • separation
  • protein
  • simulated moving bed
  • SMB

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