Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol

M Bollok, K Reczey, Guido Zacchi

Research output: Contribution to journalArticlepeer-review


Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of substrate, at a cellulase loading of 37 filter paper units/g of cellulose, and a beta-glucosidase loading of 38 IU/ g of cellulose. The detoxification of the substrate and the lack of pH control in the experiments increased the final ethanol concentration. The final ethanol yield was 15% lower compared to SSF with Saccharomyces cerevisiae at 37 degrees C, owing to the cessation of ethanol fermentation after the first 10 h.
Original languageEnglish
Pages (from-to)69-80
JournalApplied Biochemistry and Biotechnology
Publication statusPublished - 2000

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

Free keywords

  • simultaneous saccharification and fermentation
  • Kluyveromyces marxianus
  • ethanol fermentation
  • steam-pretreated spruce


Dive into the research topics of 'Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol'. Together they form a unique fingerprint.

Cite this