Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination

Katarzyna Dziarkowska, Jan Åke Jönsson, Piotr P Wieczorek

Research output: Contribution to journalArticlepeer-review

32 Citations (SciVal)

Abstract

Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma.
Original languageEnglish
Pages (from-to)184-193
JournalAnalytica Chimica Acta
Volume606
Issue number2
DOIs
Publication statusPublished - 2008

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry

Keywords

  • polyamines
  • extraction technique
  • hollow fiber membrane
  • supported liquid membrane

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