Single-step partitioning in aqueous two-phase systems

Telma Teixeira Franco; Rajni Hatti-Kaul

doi:10.1385/1-59259-028-4:47

Single-step partitioning in aqueous two-phase systems

Telma Teixeira Franco, Rajni Hatti-Kaul

Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Partitioning in aqueous two-phase system (ATPS) provides a rapid and gentle means of separation of soluble as well as particulate biomaterials, e.g. proteins, nucleic acids, cells, viruses, organelles, and membranes. Partitioning between the two phases is a complex phenomenon, guided mainly by the interaction of the partitioned substance and the phase components, e.g., through hydrogen bonds, van der Waals-, hydrophobic-, and electrostatic- interactions, steric-, and conformational effects, etc.

Original language	English
Title of host publication	Aqueous Two-Phase Systems – Methods and Protocols
Editors	Rajni Hatti-Kaul
Place of Publication	New Jersey
Publisher	Humana Press
Chapter	5
Pages	47-54
ISBN (Electronic)	978-1-59259-028-5
ISBN (Print)	978-0-89603-541-6, 978-1-61737-067-0
DOIs	https://doi.org/10.1385/1-59259-028-4:47
Publication status	Published - 2000

Subject classification (UKÄ)

Industrial Biotechnology

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10.1385/1-59259-028-4:47

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DO - 10.1385/1-59259-028-4:47

M3 - Book chapter

SN - 978-0-89603-541-6

SN - 978-1-61737-067-0

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BT - Aqueous Two-Phase Systems – Methods and Protocols

A2 - Hatti-Kaul, Rajni

PB - Humana Press

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ER -

Single-step partitioning in aqueous two-phase systems

Abstract

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