Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

J Luo; Mats Leeman; A Ballagi; A Elfwing; ZG Su; JC Janson; Karl-Gustav Wahlund

doi:10.1016/j.chroma.2005.11.048

Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

J Luo, Mats Leeman, A Ballagi, A Elfwing, ZG Su, JC Janson, Karl-Gustav Wahlund

Centre for Analysis and Synthesis

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.

Original language	English
Title of host publication	Journal of Chromatography A
Publisher	Elsevier
Pages	158-164
Volume	1120
DOIs	https://doi.org/10.1016/j.chroma.2005.11.048
Publication status	Published - 2006
Event	29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II - Stockholm, Sweden Duration: 2005 Jun 26 → 2005 Jun 30

Publication series

Name
Number	1-2
Volume	1120
ISSN (Print)	0021-9673

Conference

Conference	29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II
Country/Territory	Sweden
City	Stockholm
Period	2005/06/26 → 2005/06/30

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

Analytical Chemistry

Free keywords

hydrodynamic diameter
fluorescence protein inclusion bodies
green
multi-angle light
asymmetrical flow field-flow fractionation
scattering
root mean square radius

Access to Document

10.1016/j.chroma.2005.11.048

Cite this

@inproceedings{1ec94f7c339744f3b1f9c48dfff87612,

title = "Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering",

abstract = "The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.",

keywords = "hydrodynamic diameter, fluorescence protein inclusion bodies, green, multi-angle light, asymmetrical flow field-flow fractionation, scattering, root mean square radius",

author = "J Luo and Mats Leeman and A Ballagi and A Elfwing and ZG Su and JC Janson and Karl-Gustav Wahlund",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004); 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II ; Conference date: 26-06-2005 Through 30-06-2005",

year = "2006",

doi = "10.1016/j.chroma.2005.11.048",

language = "English",

volume = "1120",

publisher = "Elsevier",

number = "1-2",

pages = "158--164",

booktitle = "Journal of Chromatography A",

address = "United States",

}

Luo, J, Leeman, M, Ballagi, A, Elfwing, A, Su, ZG, Janson, JC & Wahlund, K-G 2006, Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering. in Journal of Chromatography A. vol. 1120, Elsevier, pp. 158-164, 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II, Stockholm, Sweden, 2005/06/26. https://doi.org/10.1016/j.chroma.2005.11.048

Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering. / Luo, J; Leeman, Mats; Ballagi, A et al.
Journal of Chromatography A. Vol. 1120 Elsevier, 2006. p. 158-164.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

AU - Luo, J

AU - Leeman, Mats

AU - Ballagi, A

AU - Elfwing, A

AU - Su, ZG

AU - Janson, JC

AU - Wahlund, Karl-Gustav

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2006

Y1 - 2006

N2 - The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.

AB - The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.

KW - hydrodynamic diameter

KW - fluorescence protein inclusion bodies

KW - green

KW - multi-angle light

KW - asymmetrical flow field-flow fractionation

KW - scattering

KW - root mean square radius

U2 - 10.1016/j.chroma.2005.11.048

DO - 10.1016/j.chroma.2005.11.048

M3 - Paper in conference proceeding

VL - 1120

SP - 158

EP - 164

BT - Journal of Chromatography A

PB - Elsevier

T2 - 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques - Part II

Y2 - 26 June 2005 through 30 June 2005

ER -

Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

Abstract

Publication series

Conference

Bibliographical note

Subject classification (UKÄ)

Free keywords

Access to Document

Fingerprint

Cite this