Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples

S. W. Lee, J. Y. Ahn, K. Järås, H. Lilja, M. J. Jo, C. Y. Jung, O. C. Jeong, S. Y. Kim, T. Laurell

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

Abstract

In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages800-802
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period2010/10/032010/10/07

Subject classification (UKÄ)

  • Medicinal Chemistry

Free keywords

  • Porous silicon
  • Sol-gel microarray. PSA

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