Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

Magdalena Kurek; Elisabet Åkesson; Masahito Yoshihara; Elizabeth Oliver; Yanhua Cui; Martin Becker; João Pedro Alves-Lopes; Ragnar Bjarnason; Patrik Romerius; Mikael Sundin; Ulrika Norén Nyström; Cecilia Langenskiöld; Hartmut Vogt; Lars Henningsohn; Cecilia Petersen; Olle Söder; Jingtao Guo; Rod T. Mitchell; Kirsi Jahnukainen; Jan Bernd Stukenborg

doi:10.3390/cells10020241

Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

Magdalena Kurek, Elisabet Åkesson, Masahito Yoshihara, Elizabeth Oliver, Yanhua Cui, Martin Becker, João Pedro Alves-Lopes, Ragnar Bjarnason, Patrik Romerius, Mikael Sundin, Ulrika Norén Nyström, Cecilia Langenskiöld, Hartmut Vogt, Lars Henningsohn, Cecilia Petersen, Olle Söder, Jingtao Guo, Rod T. Mitchell, Kirsi Jahnukainen, Jan Bernd Stukenborg

Pathways of cancer cell evolution

Research output: Contribution to journal › Article › peer-review

Abstract

Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

Original language	English
Article number	241
Journal	Cells
Volume	10
Issue number	2
DOIs	https://doi.org/10.3390/cells10020241
Publication status	Published - 2021

Subject classification (UKÄ)

Gynaecology, Obstetrics and Reproductive Medicine
Cancer and Oncology

Free keywords

basal membrane
germ cells
infertility
late effects
seminiferous tubules
Sertoli cells
spermatogonia
stem cell niche

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/cells10020241

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Kurek, M., Åkesson, E., Yoshihara, M., Oliver, E., Cui, Y., Becker, M., Alves-Lopes, J. P., Bjarnason, R., Romerius, P., Sundin, M., Norén Nyström, U., Langenskiöld, C., Vogt, H., Henningsohn, L., Petersen, C., Söder, O., Guo, J., Mitchell, R. T., Jahnukainen, K., & Stukenborg, J. B. (2021). Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells, 10(2), Article 241. https://doi.org/10.3390/cells10020241

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title = "Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation",

abstract = "Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.",

keywords = "basal membrane, germ cells, infertility, late effects, seminiferous tubules, Sertoli cells, spermatogonia, stem cell niche",

author = "Magdalena Kurek and Elisabet {\AA}kesson and Masahito Yoshihara and Elizabeth Oliver and Yanhua Cui and Martin Becker and Alves-Lopes, {Jo{\~a}o Pedro} and Ragnar Bjarnason and Patrik Romerius and Mikael Sundin and {Nor{\'e}n Nystr{\"o}m}, Ulrika and Cecilia Langenski{\"o}ld and Hartmut Vogt and Lars Henningsohn and Cecilia Petersen and Olle S{\"o}der and Jingtao Guo and Mitchell, {Rod T.} and Kirsi Jahnukainen and Stukenborg, {Jan Bernd}",

year = "2021",

doi = "10.3390/cells10020241",

language = "English",

volume = "10",

journal = "Cells",

issn = "2073-4409",

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}

Kurek, M, Åkesson, E, Yoshihara, M, Oliver, E, Cui, Y, Becker, M, Alves-Lopes, JP, Bjarnason, R, Romerius, P, Sundin, M, Norén Nyström, U, Langenskiöld, C, Vogt, H, Henningsohn, L, Petersen, C, Söder, O, Guo, J, Mitchell, RT, Jahnukainen, K & Stukenborg, JB 2021, 'Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation', Cells, vol. 10, no. 2, 241. https://doi.org/10.3390/cells10020241

TY - JOUR

T1 - Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

AU - Kurek, Magdalena

AU - Åkesson, Elisabet

AU - Yoshihara, Masahito

AU - Oliver, Elizabeth

AU - Cui, Yanhua

AU - Becker, Martin

AU - Alves-Lopes, João Pedro

AU - Bjarnason, Ragnar

AU - Romerius, Patrik

AU - Sundin, Mikael

AU - Norén Nyström, Ulrika

AU - Langenskiöld, Cecilia

AU - Vogt, Hartmut

AU - Henningsohn, Lars

AU - Petersen, Cecilia

AU - Söder, Olle

AU - Guo, Jingtao

AU - Mitchell, Rod T.

AU - Jahnukainen, Kirsi

AU - Stukenborg, Jan Bernd

PY - 2021

Y1 - 2021

N2 - Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

AB - Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

KW - basal membrane

KW - germ cells

KW - infertility

KW - late effects

KW - seminiferous tubules

KW - Sertoli cells

KW - spermatogonia

KW - stem cell niche

U2 - 10.3390/cells10020241

DO - 10.3390/cells10020241

M3 - Article

C2 - 33513766

AN - SCOPUS:85100612669

SN - 2073-4409

VL - 10

JO - Cells

JF - Cells

IS - 2

M1 - 241

ER -

Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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