Spinal Cord FAAH in Normal Micturition Control and Bladder Overactivity in Awake Rats

Claudius Fuellhase, Andrea Russo, Fabio Castiglione, Fabio Benigni, Lysanne Campeau, Francesco Montorsi, Christian Gratzke, Arianna Bettiga, Christian Stief, Karl-Erik Andersson, Petter Hedlund

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.
Original languageEnglish
Pages (from-to)2364-2370
JournalJournal of Urology
Volume189
Issue number6
DOIs
Publication statusPublished - 2013

Subject classification (UKÄ)

  • Urology and Nephrology

Free keywords

  • fatty-acid amide hydrolase
  • endocannabinoids
  • urodynamics
  • overactive
  • urinary bladder
  • urethral obstruction

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