Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Susanne Linde; Bruno Hansen; Åke Lernmark

doi:10.1016/0003-2697(80)90507-2

Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Susanne Linde, Bruno Hansen, Åke Lernmark

Hagedorn Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H₂O₂ and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method ¹²⁵I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

Original language	English
Pages (from-to)	165-176
Number of pages	12
Journal	Analytical Biochemistry
Volume	107
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90507-2
Publication status	Published - 1980 Jan 1
Externally published	Yes

Subject classification (UKÄ)

Endocrinology and Diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0003-2697(80)90507-2

Cite this

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abstract = "Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.",

author = "Susanne Linde and Bruno Hansen and {\AA}ke Lernmark",

year = "1980",

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doi = "10.1016/0003-2697(80)90507-2",

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AU - Hansen, Bruno

AU - Lernmark, Åke

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AB - Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

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DO - 10.1016/0003-2697(80)90507-2

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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ER -

Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Abstract

Subject classification (UKÄ)

UN SDGs

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