Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Susanne Linde; Bruno Hansen; Åke Lernmark

doi:10.1016/0003-2697(80)90507-2

Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Susanne Linde, Bruno Hansen, Åke Lernmark

Hagedorn Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H₂O₂ and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method ¹²⁵I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

Original language	English
Pages (from-to)	165-176
Number of pages	12
Journal	Analytical Biochemistry
Volume	107
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90507-2
Publication status	Published - 1980 Jan 1
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Subject classification (UKÄ)

Endocrinology and Diabetes

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10.1016/0003-2697(80)90507-2

Cite this

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title = "Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis",

abstract = "Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.",

author = "Susanne Linde and Bruno Hansen and {\AA}ke Lernmark",

year = "1980",

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doi = "10.1016/0003-2697(80)90507-2",

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AU - Linde, Susanne

AU - Hansen, Bruno

AU - Lernmark, Åke

PY - 1980/1/1

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N2 - Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

AB - Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

UR - https://www.scopus.com/pages/publications/0019167449

U2 - 10.1016/0003-2697(80)90507-2

DO - 10.1016/0003-2697(80)90507-2

M3 - Article

C2 - 7001948

AN - SCOPUS:0019167449

SN - 0003-2697

VL - 107

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EP - 176

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Abstract

UN SDGs

Subject classification (UKÄ)

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