Standardization of Cystatin C: development of primary and secondary reference preparations.

Soren Blirup; Anders Grubb; Veronica Lindström; C Schmidt; H Althaus

doi:10.1080/00365510802150067

Standardization of Cystatin C: development of primary and secondary reference preparations.

Soren Blirup, Anders Grubb, Veronica Lindström, C Schmidt, H Althaus

Division of Clinical Chemistry and Pharmacology

Research output: Contribution to journal › Article › peer-review

Abstract

A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.

Original language	English
Pages (from-to)	67-70
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	68
Issue number	s241
DOIs	https://doi.org/10.1080/00365510802150067
Publication status	Published - 2008

Subject classification (UKÄ)

Medicinal Chemistry
Pharmacology and Toxicology

Free keywords

Primary Reference Preparation
Secondary Reference Preparation
standardization of Cystatin C
dry mass determination
Cystatin C

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10.1080/00365510802150067

http://www.ncbi.nlm.nih.gov/pubmed/18569968?dopt=Abstract

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title = "Standardization of Cystatin C: development of primary and secondary reference preparations.",

abstract = "A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.",

keywords = "Primary Reference Preparation, Secondary Reference Preparation, standardization of Cystatin C, dry mass determination, Cystatin C",

author = "Soren Blirup and Anders Grubb and Veronica Lindstr{\"o}m and C Schmidt and H Althaus",

year = "2008",

doi = "10.1080/00365510802150067",

language = "English",

volume = "68",

pages = "67--70",

journal = "Scandinavian Journal of Clinical \& Laboratory Investigation",

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TY - JOUR

T1 - Standardization of Cystatin C: development of primary and secondary reference preparations.

AU - Blirup, Soren

AU - Grubb, Anders

AU - Lindström, Veronica

AU - Schmidt, C

AU - Althaus, H

PY - 2008

Y1 - 2008

N2 - A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.

AB - A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.

KW - Primary Reference Preparation

KW - Secondary Reference Preparation

KW - standardization of Cystatin C

KW - dry mass determination

KW - Cystatin C

UR - https://www.scopus.com/pages/publications/45849097552

U2 - 10.1080/00365510802150067

DO - 10.1080/00365510802150067

M3 - Article

SN - 1502-7686

VL - 68

SP - 67

EP - 70

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - s241

ER -

Standardization of Cystatin C: development of primary and secondary reference preparations.

Abstract

Subject classification (UKÄ)

Free keywords

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