Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

Sigurbjörg Rutardottir; Elena Karnaukhova; Chanin Nantasenamat; Napat Songtawee; Virapong Prachayasittikul; Mohsen Rajabi; Lena Wester Rosenlöf; Abdu I Alayash; Bo Åkerström

doi:10.1016/j.bbapap.2015.10.002

Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

Sigurbjörg Rutardottir, Elena Karnaukhova, Chanin Nantasenamat, Napat Songtawee, Virapong Prachayasittikul, Mohsen Rajabi, Lena Wester Rosenlöf, Abdu I Alayash, Bo Åkerström

Infection Medicine (BMC)

Research output: Contribution to journal › Article › peer-review

Abstract

α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.

Original language	English
Pages (from-to)	29-41
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1864
Issue number	1
DOIs	https://doi.org/10.1016/j.bbapap.2015.10.002
Publication status	Published - 2016

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Division of Infection Medicine (BMC) (013024020), Faculty of Medicine (000022000), Medical Inflammation Research (013212019)

Subject classification (UKÄ)

Cell and Molecular Biology

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10.1016/j.bbapap.2015.10.002

http://www.ncbi.nlm.nih.gov/pubmed/26497278?dopt=Abstract

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Rutardottir, S., Karnaukhova, E., Nantasenamat, C., Songtawee, N., Prachayasittikul, V., Rajabi, M., Wester Rosenlöf, L., Alayash, A. I., & Åkerström, B. (2016). Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation. Biochimica et Biophysica Acta - Proteins and Proteomics, 1864(1), 29-41. https://doi.org/10.1016/j.bbapap.2015.10.002

@article{fdad36cc943b4ada8339bd3cbc61da06,

title = "Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.",

abstract = "α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.",

author = "Sigurbj{\"o}rg Rutardottir and Elena Karnaukhova and Chanin Nantasenamat and Napat Songtawee and Virapong Prachayasittikul and Mohsen Rajabi and {Wester Rosenl{\"o}f}, Lena and Alayash, {Abdu I} and Bo {\AA}kerstr{\"o}m",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Infection Medicine (BMC) (013024020), Faculty of Medicine (000022000), Medical Inflammation Research (013212019)",

year = "2016",

doi = "10.1016/j.bbapap.2015.10.002",

language = "English",

volume = "1864",

pages = "29--41",

journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",

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number = "1",

}

Rutardottir, S, Karnaukhova, E, Nantasenamat, C, Songtawee, N, Prachayasittikul, V, Rajabi, M, Wester Rosenlöf, L, Alayash, AI & Åkerström, B 2016, 'Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.', Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1864, no. 1, pp. 29-41. https://doi.org/10.1016/j.bbapap.2015.10.002

Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation. / Rutardottir, Sigurbjörg; Karnaukhova, Elena; Nantasenamat, Chanin et al.
In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1864, No. 1, 2016, p. 29-41.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

AU - Rutardottir, Sigurbjörg

AU - Karnaukhova, Elena

AU - Nantasenamat, Chanin

AU - Songtawee, Napat

AU - Prachayasittikul, Virapong

AU - Rajabi, Mohsen

AU - Wester Rosenlöf, Lena

AU - Alayash, Abdu I

AU - Åkerström, Bo

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Infection Medicine (BMC) (013024020), Faculty of Medicine (000022000), Medical Inflammation Research (013212019)

PY - 2016

Y1 - 2016

N2 - α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.

AB - α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.

UR - https://www.sciencedirect.com/science/article/pii/S1570963920300352?via%3Dihub

U2 - 10.1016/j.bbapap.2015.10.002

DO - 10.1016/j.bbapap.2015.10.002

M3 - Article

C2 - 26497278

SN - 1570-9639

VL - 1864

SP - 29

EP - 41

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

IS - 1

ER -

Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

Abstract

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