Study of ATP-release from acoustically levitated eryhrocytes

Mikael Evander; Karolina Mileros; Carl Högberg; David Erlinge; Monica Almqvist; Thomas Laurell; Johan Nilsson

Study of ATP-release from acoustically levitated eryhrocytes

Mikael Evander, Karolina Mileros, Carl Högberg, David Erlinge, Monica Almqvist, Thomas Laurell, Johan Nilsson

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

Erythrocytes (red blood cells) are known to produce large amounts of Adenosine Triphosphate (ATP). It has recently become clear that the ATP-release is part of a mechanism controlling the dilation of the body’s blood vessels. The study of the erythrocyte’s behaviour is complicated by the fact that they respond easily to any physical contact. In this paper we propose a new method for studying the dynamics of the ATP-release by combining non-contact acoustic trapping in a microfluidic chip with a chemiluminiscent assay. Sensitivity levels down to 10 pM were achievable and the ATP-release from a cluster of levitated live erythrocytes was recorded.

Original language	English
Title of host publication	The proceedings of micro total analysis systems 2007
Editors	Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
Publisher	Chemical and Biological Microsystems Society
Pages	1372-1374
Number of pages	3
Volume	2
ISBN (Print)	978-0-9798064-0-7
Publication status	Published - 2007
Event	Micro Total Analysis Systems 2007 - Paris Duration: 2007 Oct 7 → 2007 Oct 11

Publication series

Name
Volume	2

Conference

Conference	Micro Total Analysis Systems 2007
Period	2007/10/07 → 2007/10/11

Subject classification (UKÄ)

Medical Engineering

Free keywords

Chemiluminiscence
Erythrocytes
ATP
Acoustic trapping

1 Published meeting abstract

Study of ATP-release from acoustically levitated eryhrocytes
Evander, M., Mileros, K., Högberg, C., Erlinge, D., Almqvist, M., Laurell, T. & Nilsson, J., 2007, In: Nano2Life Autumn Meeting. 1, p. 24-24
Research output: Contribution to journal › Published meeting abstract › peer-review

Cite this

@inproceedings{74b8ec189981446c91ae14bcf7343778,

title = "Study of ATP-release from acoustically levitated eryhrocytes",

abstract = "Erythrocytes (red blood cells) are known to produce large amounts of Adenosine Triphosphate (ATP). It has recently become clear that the ATP-release is part of a mechanism controlling the dilation of the body{\textquoteright}s blood vessels. The study of the erythrocyte{\textquoteright}s behaviour is complicated by the fact that they respond easily to any physical contact. In this paper we propose a new method for studying the dynamics of the ATP-release by combining non-contact acoustic trapping in a microfluidic chip with a chemiluminiscent assay. Sensitivity levels down to 10 pM were achievable and the ATP-release from a cluster of levitated live erythrocytes was recorded.",

keywords = "Chemiluminiscence, Erythrocytes, ATP, Acoustic trapping",

author = "Mikael Evander and Karolina Mileros and Carl H{\"o}gberg and David Erlinge and Monica Almqvist and Thomas Laurell and Johan Nilsson",

year = "2007",

language = "English",

isbn = "978-0-9798064-0-7",

volume = "2",

publisher = "Chemical and Biological Microsystems Society",

pages = "1372--1374",

editor = "Jean-Louis Viovy and Patrick Tabeling and Stephanie Descroix and Laurent Malaquin",

booktitle = "The proceedings of micro total analysis systems 2007",

note = "Micro Total Analysis Systems 2007 ; Conference date: 07-10-2007 Through 11-10-2007",

}

Evander, M, Mileros, K, Högberg, C, Erlinge, D , Almqvist, M , Laurell, T & Nilsson, J 2007, Study of ATP-release from acoustically levitated eryhrocytes. in J-L Viovy, P Tabeling, S Descroix & L Malaquin (eds), The proceedings of micro total analysis systems 2007. vol. 2, Chemical and Biological Microsystems Society, pp. 1372-1374, Micro Total Analysis Systems 2007, 2007/10/07.

Study of ATP-release from acoustically levitated eryhrocytes. / Evander, Mikael; Mileros, Karolina; Högberg, Carl et al.
The proceedings of micro total analysis systems 2007. ed. / Jean-Louis Viovy; Patrick Tabeling; Stephanie Descroix; Laurent Malaquin. Vol. 2 Chemical and Biological Microsystems Society, 2007. p. 1372-1374.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Study of ATP-release from acoustically levitated eryhrocytes

AU - Evander, Mikael

AU - Mileros, Karolina

AU - Högberg, Carl

AU - Erlinge, David

AU - Almqvist, Monica

AU - Laurell, Thomas

AU - Nilsson, Johan

PY - 2007

Y1 - 2007

N2 - Erythrocytes (red blood cells) are known to produce large amounts of Adenosine Triphosphate (ATP). It has recently become clear that the ATP-release is part of a mechanism controlling the dilation of the body’s blood vessels. The study of the erythrocyte’s behaviour is complicated by the fact that they respond easily to any physical contact. In this paper we propose a new method for studying the dynamics of the ATP-release by combining non-contact acoustic trapping in a microfluidic chip with a chemiluminiscent assay. Sensitivity levels down to 10 pM were achievable and the ATP-release from a cluster of levitated live erythrocytes was recorded.

AB - Erythrocytes (red blood cells) are known to produce large amounts of Adenosine Triphosphate (ATP). It has recently become clear that the ATP-release is part of a mechanism controlling the dilation of the body’s blood vessels. The study of the erythrocyte’s behaviour is complicated by the fact that they respond easily to any physical contact. In this paper we propose a new method for studying the dynamics of the ATP-release by combining non-contact acoustic trapping in a microfluidic chip with a chemiluminiscent assay. Sensitivity levels down to 10 pM were achievable and the ATP-release from a cluster of levitated live erythrocytes was recorded.

KW - Chemiluminiscence

KW - Erythrocytes

KW - ATP

KW - Acoustic trapping

M3 - Paper in conference proceeding

SN - 978-0-9798064-0-7

VL - 2

SP - 1372

EP - 1374

BT - The proceedings of micro total analysis systems 2007

A2 - Viovy, Jean-Louis

A2 - Tabeling, Patrick

A2 - Descroix, Stephanie

A2 - Malaquin, Laurent

PB - Chemical and Biological Microsystems Society

T2 - Micro Total Analysis Systems 2007

Y2 - 7 October 2007 through 11 October 2007

ER -

Study of ATP-release from acoustically levitated eryhrocytes

Abstract

Publication series

Conference

Subject classification (UKÄ)

Free keywords

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Research output

Study of ATP-release from acoustically levitated eryhrocytes

Cite this