Studying the innate immune response to myocardial infarction in a highly efficient experimental animal model

Razvan Gheorghita Mares, Doina Manu, Istvan Adorjan Szabo, Mihaela Elena Tomut, Gabriela Pintican, Bogdan Cordos, Gabriel Jakobsson, Minodora Dobreanu, Ovidiu Simion Cotoi, Alexandru Schiopu

Research output: Contribution to journalArticlepeer-review

Abstract

The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has attracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary artery (LAD) in mice, followed by complex flow-cytometry and histological analyses of immune cellular populations in blood and myocardium. We are presenting time-dependent qualitative and quantitative analysis results, demonstrating intense accumulation of Ly6Ghi neutrophils and Ly6Chi monocytes in the infarcted myocardium on days 1 and 3 post-AMI, followed by successive accumulation of reparatory Ly6CloMerTKhi macrophages, neovascularization and fibrosis development by day 7.

Original languageEnglish
Pages (from-to)573-585
JournalRevista Romana de Cardiologie
Volume31
Issue number3
DOIs
Publication statusPublished - 2021

Subject classification (UKÄ)

  • Cardiac and Cardiovascular Systems

Free keywords

  • Acute myocardial infarction
  • Experimental model
  • Innate immunity
  • Macrophages
  • Monocytes
  • Myocardial fibrosis
  • Neutrophils
  • Revascularization

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