Subcellular distribution of spermidine/spermine N(1)-acetyltransferase

Martina Holst; Pernilla Nevsten; Fredrik I Johansson; Eric Carlemalm; Stina Oredsson

doi:10.1016/j.cellbi.2007.08.008

Subcellular distribution of spermidine/spermine N(1)-acetyltransferase

Martina Holst, Pernilla Nevsten, Fredrik I Johansson, Eric Carlemalm, Stina Oredsson

Research output: Contribution to journal › Article › peer-review

Abstract

The subcellular distribution of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was studied in L56Br-C1 cells treated with 10muM N(1),N(11)-diethylnorspermine (DENSPM) for 24h. Cells were fractioned into three subcellular fractions. A particulate fraction containing the mitochondria was denoted as the mitochondrial fraction. After DENSPM treatment, an increase in SSAT activity was mainly found in the mitochondrial fraction. Western blot analysis showed an increased level of the SSAT protein in the mitochondrial fraction compared to the cytosolic fraction. Immunofluorescence microscopy and immunogold labeling transmission electron microscopy also showed a mitochondrial association of SSAT. Transmission electron microscopy revealed that the endoplasmic reticulum was devoid of ribosomes in DENSPM-treated cells, in contrast to control cells that contained ample ribosomes. An increased SSAT activity in connection with the mitochondria may be part of the mechanism of DENSPM-induced apoptosis.

Original language	English
Pages (from-to)	39-47
Journal	Cell Biology International
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/j.cellbi.2007.08.008
Publication status	Published - 2008

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100), Lund University Bioimaging Center (013100101), Polymer and Materials Chemistry (LTH) (011001041)

Subject classification (UKÄ)

Zoology

Access to Document

10.1016/j.cellbi.2007.08.008

1 Doctoral Thesis (compilation)

Development and application of advanced electron microscopy techniques for materials in biological systems
Nevsten, P., 2008
Research output: Thesis › Doctoral Thesis (compilation)

Cite this

@article{e4fcea11d3314fa7831d262e900a72ad,

title = "Subcellular distribution of spermidine/spermine N(1)-acetyltransferase",

abstract = "The subcellular distribution of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was studied in L56Br-C1 cells treated with 10muM N(1),N(11)-diethylnorspermine (DENSPM) for 24h. Cells were fractioned into three subcellular fractions. A particulate fraction containing the mitochondria was denoted as the mitochondrial fraction. After DENSPM treatment, an increase in SSAT activity was mainly found in the mitochondrial fraction. Western blot analysis showed an increased level of the SSAT protein in the mitochondrial fraction compared to the cytosolic fraction. Immunofluorescence microscopy and immunogold labeling transmission electron microscopy also showed a mitochondrial association of SSAT. Transmission electron microscopy revealed that the endoplasmic reticulum was devoid of ribosomes in DENSPM-treated cells, in contrast to control cells that contained ample ribosomes. An increased SSAT activity in connection with the mitochondria may be part of the mechanism of DENSPM-induced apoptosis.",

author = "Martina Holst and Pernilla Nevsten and Johansson, {Fredrik I} and Eric Carlemalm and Stina Oredsson",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100), Lund University Bioimaging Center (013100101), Polymer and Materials Chemistry (LTH) (011001041)",

year = "2008",

doi = "10.1016/j.cellbi.2007.08.008",

language = "English",

volume = "32",

pages = "39--47",

journal = "Cell Biology International",

issn = "1095-8355",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Subcellular distribution of spermidine/spermine N(1)-acetyltransferase

AU - Holst, Martina

AU - Nevsten, Pernilla

AU - Johansson, Fredrik I

AU - Carlemalm, Eric

AU - Oredsson, Stina

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100), Lund University Bioimaging Center (013100101), Polymer and Materials Chemistry (LTH) (011001041)

PY - 2008

Y1 - 2008

N2 - The subcellular distribution of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was studied in L56Br-C1 cells treated with 10muM N(1),N(11)-diethylnorspermine (DENSPM) for 24h. Cells were fractioned into three subcellular fractions. A particulate fraction containing the mitochondria was denoted as the mitochondrial fraction. After DENSPM treatment, an increase in SSAT activity was mainly found in the mitochondrial fraction. Western blot analysis showed an increased level of the SSAT protein in the mitochondrial fraction compared to the cytosolic fraction. Immunofluorescence microscopy and immunogold labeling transmission electron microscopy also showed a mitochondrial association of SSAT. Transmission electron microscopy revealed that the endoplasmic reticulum was devoid of ribosomes in DENSPM-treated cells, in contrast to control cells that contained ample ribosomes. An increased SSAT activity in connection with the mitochondria may be part of the mechanism of DENSPM-induced apoptosis.

AB - The subcellular distribution of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was studied in L56Br-C1 cells treated with 10muM N(1),N(11)-diethylnorspermine (DENSPM) for 24h. Cells were fractioned into three subcellular fractions. A particulate fraction containing the mitochondria was denoted as the mitochondrial fraction. After DENSPM treatment, an increase in SSAT activity was mainly found in the mitochondrial fraction. Western blot analysis showed an increased level of the SSAT protein in the mitochondrial fraction compared to the cytosolic fraction. Immunofluorescence microscopy and immunogold labeling transmission electron microscopy also showed a mitochondrial association of SSAT. Transmission electron microscopy revealed that the endoplasmic reticulum was devoid of ribosomes in DENSPM-treated cells, in contrast to control cells that contained ample ribosomes. An increased SSAT activity in connection with the mitochondria may be part of the mechanism of DENSPM-induced apoptosis.

U2 - 10.1016/j.cellbi.2007.08.008

DO - 10.1016/j.cellbi.2007.08.008

M3 - Article

SN - 1095-8355

VL - 32

SP - 39

EP - 47

JO - Cell Biology International

JF - Cell Biology International

IS - 1

ER -

Subcellular distribution of spermidine/spermine N(1)-acetyltransferase

Abstract

Bibliographical note

Subject classification (UKÄ)

Access to Document

Fingerprint

Research output

Development and application of advanced electron microscopy techniques for materials in biological systems

Cite this