Substance P relaxes rat bronchial smooth muscle via epithelial prostanoid synthesis

Mikael Bodelsson, Sten Blomquist, Kaj Caverius, Kenneth Tornebrandt

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Substance P is present in bronchial nerve fibres. The physiological actions of substance P are mediated via tachykinin NK(1) receptors. Immunochemical studies have demonstrated tachykinin NK(1) receptors in the rat airway epithelium. OBJECTIVE: To elucidate how epithelial tachykinin NK(1) receptors affect smooth muscle response to substance P. METHODS: Contractile response of isolated rat bronchial trunk with or without epithelium was recorded. RESULTS: In intact segments precontracted by 5-hydroxytryptamine, relaxation was induced by substance P and the nitric oxide donor, sodium nitroprusside. Removal of the epithelium abolished relaxation induced by substance P but did not affect relaxation induced by sodium nitroprusside. The cyclo-oxygenase inhibitor, indomethacin, but not the nitric oxide synthase inhibitor, L-N(G)-monomethylarginine, reduced the relaxation in response to substance P. CONCLUSIONS: Epithelial tachykinin NK(1) receptors mediate substance-P-induced relaxation of rat bronchial smooth muscle via release of prostanoids but not nitric oxide.
Original languageEnglish
Pages (from-to)355-359
JournalRespiration
Volume66
Issue number4
DOIs
Publication statusPublished - 1999

Subject classification (UKÄ)

  • Respiratory Medicine and Allergy

Keywords

  • Bronchi
  • Epithelium
  • Indomethacin
  • Nitric oxide
  • Serotonin
  • Substance P

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