Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

U H Stenman; E Paus; W J Allard; I Andersson; C Andres; T R Barnett; Charlotte Becker; A Belenky; L Bellanger; C M Pellegrino; O P Bormer; G Davis; B Dowell; L S Grauer; D C Jette; B Karlsson; F T Kreutz; T M van der Kwast; L Lauren; M Leinimaa; J Leinonen; Hans Lilja; H J Linton; M Nap; O Nilsson; P C Ng; K Nustad; A Peter; K Pettersson; T Piironen; J Rapp; H G Rittenhouse; P D Rye; P Seguin; J Slota; R L Sokoloff; M R Suresh; D L Very; T J Wang; Ingrid Wigheden; R L Wolfert; K K Yeung; W-M Zhang; Z Zhou; J Hilgers

doi:10.1159/000056523

Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

U H Stenman, E Paus, W J Allard, I Andersson, C Andres, T R Barnett, Charlotte Becker, A Belenky, L Bellanger, C M Pellegrino, O P Bormer, G Davis, B Dowell, L S Grauer, D C Jette, B Karlsson, F T Kreutz, T M van der Kwast, L Lauren, M LeinimaaJ Leinonen, Hans Lilja, H J Linton, M Nap, O Nilsson, P C Ng, K Nustad, A Peter, K Pettersson, T Piironen, J Rapp, H G Rittenhouse, P D Rye, P Seguin, J Slota, R L Sokoloff, M R Suresh, D L Very, T J Wang, Ingrid Wigheden, R L Wolfert, K K Yeung, W-M Zhang, Z Zhou, J Hilgers

Research output: Contribution to journal › Article › peer-review

Abstract

Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.

Original language	English
Pages (from-to)	1-12
Journal	Tumor Biology
Volume	20
Issue number	Suppl. 1
DOIs	https://doi.org/10.1159/000056523
Publication status	Published - 1999

Subject classification (UKÄ)

Cancer and Oncology

Keywords

PSA
Prostate
Antibody
Workshop
ISOBM

Access to Document

10.1159/000056523

Cite this

Stenman, U. H., Paus, E., Allard, W. J., Andersson, I., Andres, C., Barnett, T. R., Becker, C., Belenky, A., Bellanger, L., Pellegrino, C. M., Bormer, O. P., Davis, G., Dowell, B., Grauer, L. S., Jette, D. C., Karlsson, B., Kreutz, F. T., van der Kwast, T. M., Lauren, L., ... Hilgers, J. (1999). Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen. Tumor Biology, 20(Suppl. 1), 1-12. https://doi.org/10.1159/000056523

@article{dd52b522f26f4f81a0d7cf93c0ba2804,

title = "Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen",

abstract = "Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.",

keywords = "PSA, Prostate, Antibody, Workshop, ISOBM",

author = "Stenman, {U H} and E Paus and Allard, {W J} and I Andersson and C Andres and Barnett, {T R} and Charlotte Becker and A Belenky and L Bellanger and Pellegrino, {C M} and Bormer, {O P} and G Davis and B Dowell and Grauer, {L S} and Jette, {D C} and B Karlsson and Kreutz, {F T} and {van der Kwast}, {T M} and L Lauren and M Leinimaa and J Leinonen and Hans Lilja and Linton, {H J} and M Nap and O Nilsson and Ng, {P C} and K Nustad and A Peter and K Pettersson and T Piironen and J Rapp and Rittenhouse, {H G} and Rye, {P D} and P Seguin and J Slota and Sokoloff, {R L} and Suresh, {M R} and Very, {D L} and Wang, {T J} and Ingrid Wigheden and Wolfert, {R L} and Yeung, {K K} and W-M Zhang and Z Zhou and J Hilgers",

year = "1999",

doi = "10.1159/000056523",

language = "English",

volume = "20",

pages = "1--12",

journal = "Tumor Biology",

issn = "1423-0380",

publisher = "Springer",

number = "Suppl. 1",

}

Stenman, UH, Paus, E, Allard, WJ, Andersson, I, Andres, C, Barnett, TR, Becker, C, Belenky, A, Bellanger, L, Pellegrino, CM, Bormer, OP, Davis, G, Dowell, B, Grauer, LS, Jette, DC, Karlsson, B, Kreutz, FT, van der Kwast, TM, Lauren, L, Leinimaa, M, Leinonen, J, Lilja, H, Linton, HJ, Nap, M, Nilsson, O, Ng, PC, Nustad, K, Peter, A, Pettersson, K, Piironen, T, Rapp, J, Rittenhouse, HG, Rye, PD, Seguin, P, Slota, J, Sokoloff, RL, Suresh, MR, Very, DL, Wang, TJ, Wigheden, I, Wolfert, RL, Yeung, KK, Zhang, W-M, Zhou, Z & Hilgers, J 1999, 'Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen', Tumor Biology, vol. 20, no. Suppl. 1, pp. 1-12. https://doi.org/10.1159/000056523

TY - JOUR

T1 - Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

AU - Stenman, U H

AU - Paus, E

AU - Allard, W J

AU - Andersson, I

AU - Andres, C

AU - Barnett, T R

AU - Becker, Charlotte

AU - Belenky, A

AU - Bellanger, L

AU - Pellegrino, C M

AU - Bormer, O P

AU - Davis, G

AU - Dowell, B

AU - Grauer, L S

AU - Jette, D C

AU - Karlsson, B

AU - Kreutz, F T

AU - van der Kwast, T M

AU - Lauren, L

AU - Leinimaa, M

AU - Leinonen, J

AU - Lilja, Hans

AU - Linton, H J

AU - Nap, M

AU - Nilsson, O

AU - Ng, P C

AU - Nustad, K

AU - Peter, A

AU - Pettersson, K

AU - Piironen, T

AU - Rapp, J

AU - Rittenhouse, H G

AU - Rye, P D

AU - Seguin, P

AU - Slota, J

AU - Sokoloff, R L

AU - Suresh, M R

AU - Very, D L

AU - Wang, T J

AU - Wigheden, Ingrid

AU - Wolfert, R L

AU - Yeung, K K

AU - Zhang, W-M

AU - Zhou, Z

AU - Hilgers, J

PY - 1999

Y1 - 1999

N2 - Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.

AB - Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.

KW - PSA

KW - Prostate

KW - Antibody

KW - Workshop

KW - ISOBM

U2 - 10.1159/000056523

DO - 10.1159/000056523

M3 - Article

VL - 20

SP - 1

EP - 12

JO - Tumor Biology

JF - Tumor Biology

SN - 1423-0380

IS - Suppl. 1

ER -

Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

Abstract

Subject classification (UKÄ)

Keywords

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