Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

U H Stenman, E Paus, W J Allard, I Andersson, C Andres, T R Barnett, Charlotte Becker, A Belenky, L Bellanger, C M Pellegrino, O P Bormer, G Davis, B Dowell, L S Grauer, D C Jette, B Karlsson, F T Kreutz, T M van der Kwast, L Lauren, M LeinimaaJ Leinonen, Hans Lilja, H J Linton, M Nap, O Nilsson, P C Ng, K Nustad, A Peter, K Pettersson, T Piironen, J Rapp, H G Rittenhouse, P D Rye, P Seguin, J Slota, R L Sokoloff, M R Suresh, D L Very, T J Wang, Ingrid Wigheden, R L Wolfert, K K Yeung, W-M Zhang, Z Zhou, J Hilgers

Research output: Contribution to journalArticlepeer-review

Abstract

Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
Original languageEnglish
Pages (from-to)1-12
JournalTumor Biology
Volume20
Issue numberSuppl. 1
DOIs
Publication statusPublished - 1999

Subject classification (UKÄ)

  • Cancer and Oncology

Free keywords

  • PSA
  • Prostate
  • Antibody
  • Workshop
  • ISOBM

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