TY - JOUR
T1 - Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument
AU - Steiner, Barbara
AU - Winter, Christine
AU - Blumensath, Stefan
AU - Paul, Gesine
AU - Harnack, Daniel
AU - Nikkhah, Guido
AU - Kupsch, Andreas
PY - 2008/3/30
Y1 - 2008/3/30
N2 - Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.
AB - Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.
KW - Cannula size
KW - Human dopaminergic cells
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=39749112525&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2007.11.032
DO - 10.1016/j.jneumeth.2007.11.032
M3 - Article
C2 - 18241930
AN - SCOPUS:39749112525
SN - 0165-0270
VL - 169
SP - 128
EP - 134
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -