Survival-associated heterogeneity of marker-defined perivascular cells in colorectal cancer

Artur Mezheyeuski, Maja Bradic Lindh, Tormod Kyrre Guren, Anca Dragomir, Per Pfeiffer, Elin H Kure, Tone Ikdahl, Eva Skovlund, Sara Corvigno, Carina Strell, Kristian Pietras, Fredrik Ponten, Jan Mulder, Camilla Qvortrup, Anna Portyanko, Kjell Magne Tveit, Bengt Glimelius, Halfdan Sorbye, Arne Östman

Research output: Contribution to journalArticlepeer-review


Perivascular cells (PC) were recently implied as regulators of metastasis and immune cell activity. Perivascular heterogeneity in clinical samples, and associations with other tumor features and outcome, remain largely unknown.Here we report a novel method for digital quantitative analyses of vessel characteristics and PC, which was applied to two collections of human metastatic colorectal cancer (mCRC).Initial analyses identified marker-defined subsets of PC, including cells expressing PDGFR-β or α-SMA or both markers. PC subsets were largely independently expressed in a manner unrelated to vessel density and size. Association studies implied specific oncogenic mutations in malignant cells as determinants of PC status. Semi-quantitative and digital-image-analyses-based scoring of the NORDIC-VII cohort identified significant associations between low expression of perivascular PDGFR-α and -β and shorter overall survival. Analyses of the SPCRC cohort confirmed these findings. Perivascular PDGFR-α and -β remained independent factors for survival in multivariate analyses.Overall, our study identified host vasculature and oncogenic status as determinants of tumor perivascular features. Perivascular PDGFR-α and -β were identified as novel independent markers predicting survival in mCRC. The novel methodology should be suitable for similar analyses in other tumor collections.

Original languageEnglish
Pages (from-to)41948-41958
Number of pages11
Issue number27
Publication statusPublished - 2016 Jul 5

Subject classification (UKÄ)

  • Cancer and Oncology

Free keywords

  • Actins/metabolism
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antineoplastic Combined Chemotherapy Protocols/therapeutic use
  • Biomarkers, Tumor/metabolism
  • Cohort Studies
  • Colorectal Neoplasms/blood supply
  • Female
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Neoplasm Metastasis
  • Pericytes/metabolism
  • Receptor, Platelet-Derived Growth Factor alpha/metabolism
  • Receptor, Platelet-Derived Growth Factor beta/metabolism
  • Young Adult


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