Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Alfredo Gonzalez Perez; Sanja Bulut; Ulf Olsson; Björn Lindman

doi:10.1007/2882_2008_106

Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Alfredo Gonzalez Perez, Sanja Bulut, Ulf Olsson, Björn Lindman

Physical Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.

Original language	English
Title of host publication	Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)
Publisher	Springer
Pages	174-180
Volume	135
ISBN (Print)	978-3-540-85133-2
DOIs	https://doi.org/10.1007/2882_2008_106
Publication status	Published - 2008
Event	9th International Conference on Colloid Chemistry, 1997 - Siofok, Hungary Duration: 1997 Jul 6 → 1997 Jul 12 Conference number: 9

Publication series

Name
Volume	135
ISSN (Print)	0340-255X

Conference

Conference	9th International Conference on Colloid Chemistry, 1997
Country/Territory	Hungary
City	Siofok
Period	1997/07/06 → 1997/07/12

Subject classification (UKÄ)

Physical Chemistry (including Surface- and Colloid Chemistry)

Free keywords

compaction
surfactant
DNA
decompaction
Mesh phase
Nonionic
C10E3
lamellar phase

Access to Document

10.1007/2882_2008_106

Cite this

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title = "Temperature Induced DNA Compaction in a Nonionic Lamellar Phase",

abstract = "A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt \% was immobilized in a lamellar phase with 40 wt \% C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.",

keywords = "compaction, surfactant, DNA, decompaction, Mesh phase, Nonionic, C10E3, lamellar phase",

author = "\{Gonzalez Perez\}, Alfredo and Sanja Bulut and Ulf Olsson and Bj{\"o}rn Lindman",

year = "2008",

doi = "10.1007/2882\_2008\_106",

language = "English",

isbn = "978-3-540-85133-2",

volume = "135",

publisher = "Springer",

pages = "174--180",

booktitle = "Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)",

address = "Germany",

note = "9th International Conference on Colloid Chemistry, 1997 ; Conference date: 06-07-1997 Through 12-07-1997",

}

TY - GEN

T1 - Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

AU - Gonzalez Perez, Alfredo

AU - Bulut, Sanja

AU - Olsson, Ulf

AU - Lindman, Björn

N1 - Conference code: 9

PY - 2008

Y1 - 2008

N2 - A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.

AB - A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.

KW - compaction

KW - surfactant

KW - DNA

KW - decompaction

KW - Mesh phase

KW - Nonionic

KW - C10E3

KW - lamellar phase

UR - https://www.scopus.com/pages/publications/62449186768

U2 - 10.1007/2882_2008_106

DO - 10.1007/2882_2008_106

M3 - Paper in conference proceeding

SN - 978-3-540-85133-2

VL - 135

SP - 174

EP - 180

BT - Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)

PB - Springer

T2 - 9th International Conference on Colloid Chemistry, 1997

Y2 - 6 July 1997 through 12 July 1997

ER -

Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Abstract

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Subject classification (UKÄ)

Free keywords

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