Abstract

α-Synuclein is an intrinsically disordered presynaptic protein associated with Parkinson's disease. The physiological role of α-Synuclein is not fully understood, but the protein is known to interact with lipid membranes. We here study how membrane charge affects the adsorption of α-Synuclein to (i) supported lipid bilayers and (ii) small unilamellar vesicles with varying amounts of anionic lipids. The results showed that α-Synuclein adsorbs onto membranes containing ≥5% anionic phosphatidylserine (DOPS) lipids, but not to membranes containing ≤1% DOPS. The density of adsorbed α-Synuclein increased steadily with the DOPS content up to 20% DOPS, after which it leveled off. The vesicles were saturated with α-Synuclein at a 3–5 times higher protein density compared to the supported bilayers, which suggests that a more deformable membrane binds more α-Synuclein. Altogether, the results show that both membrane charge density and flexibility influence the association of α-Synuclein to lipid membranes.

Original languageEnglish
Article number107143
JournalBiophysical Chemistry
Volume305
DOIs
Publication statusPublished - 2024 Feb

Subject classification (UKÄ)

  • Biophysics
  • Physical Chemistry

Free keywords

  • Alpha-synuclein
  • Circular dichroism spectroscopy
  • Fluorescence microscopy
  • Lipid bilayer mobility
  • Protein adsorption
  • Supported lipid bilayers

Fingerprint

Dive into the research topics of 'The density of anionic lipids modulates the adsorption of α-Synuclein onto lipid membranes'. Together they form a unique fingerprint.

Cite this