The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.

Sidinh Luc; Tiago C Luis; Hanane Boukarabila; Iain C Macaulay; Natalija Buza-Vidas; Tiphaine Bouriez-Jones; Michael Lutteropp; Petter S Woll; Stephen J Loughran; Adam J Mead; Anne Hultquist; John Brown; Takuo Mizukami; Sahoko Matsuoka; Helen Ferry; Kristina Anderson; Sara Duarte; Deborah Atkinson; Shamit Soneji; Aniela Domanski; Alison Farley; Alejandra Sanjuan-Pla; Cintia Carella; Roger Patient; Marella de Bruijn; Tariq Enver; Claus Nerlov; Clare Blackburn; Isabelle Godin; Sten Eirik W Jacobsen

doi:10.1038/ni.2255

The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.

Sidinh Luc, Tiago C Luis, Hanane Boukarabila, Iain C Macaulay, Natalija Buza-Vidas, Tiphaine Bouriez-Jones, Michael Lutteropp, Petter S Woll, Stephen J Loughran, Adam J Mead, Anne Hultquist, John Brown, Takuo Mizukami, Sahoko Matsuoka, Helen Ferry, Kristina Anderson, Sara Duarte, Deborah Atkinson, Shamit Soneji, Aniela DomanskiAlison Farley, Alejandra Sanjuan-Pla, Cintia Carella, Roger Patient, Marella de Bruijn, Tariq Enver, Claus Nerlov, Clare Blackburn, Isabelle Godin, Sten Eirik W Jacobsen

Stem Cell Center

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Abstract

The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.

Original language	English
Pages (from-to)	412-419
Journal	Nature Immunology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1038/ni.2255
Publication status	Published - 2012

Subject classification (UKÄ)

Cell and Molecular Biology

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10.1038/ni.2255

http://www.ncbi.nlm.nih.gov/pubmed/22344248?dopt=Abstract

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Luc, S., Luis, T. C., Boukarabila, H., Macaulay, I. C., Buza-Vidas, N., Bouriez-Jones, T., Lutteropp, M., Woll, P. S., Loughran, S. J., Mead, A. J., Hultquist, A., Brown, J., Mizukami, T., Matsuoka, S., Ferry, H., Anderson, K., Duarte, S., Atkinson, D., Soneji, S., ... Jacobsen, S. E. W. (2012). The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential. Nature Immunology, 13(4), 412-419. https://doi.org/10.1038/ni.2255

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title = "The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.",

abstract = "The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.",

author = "Sidinh Luc and Luis, {Tiago C} and Hanane Boukarabila and Macaulay, {Iain C} and Natalija Buza-Vidas and Tiphaine Bouriez-Jones and Michael Lutteropp and Woll, {Petter S} and Loughran, {Stephen J} and Mead, {Adam J} and Anne Hultquist and John Brown and Takuo Mizukami and Sahoko Matsuoka and Helen Ferry and Kristina Anderson and Sara Duarte and Deborah Atkinson and Shamit Soneji and Aniela Domanski and Alison Farley and Alejandra Sanjuan-Pla and Cintia Carella and Roger Patient and {de Bruijn}, Marella and Tariq Enver and Claus Nerlov and Clare Blackburn and Isabelle Godin and Jacobsen, {Sten Eirik W}",

year = "2012",

doi = "10.1038/ni.2255",

language = "English",

volume = "13",

pages = "412--419",

journal = "Nature Immunology",

issn = "1529-2908",

publisher = "Nature Publishing Group",

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Luc, S, Luis, TC, Boukarabila, H, Macaulay, IC, Buza-Vidas, N, Bouriez-Jones, T, Lutteropp, M, Woll, PS, Loughran, SJ, Mead, AJ, Hultquist, A, Brown, J, Mizukami, T, Matsuoka, S, Ferry, H, Anderson, K, Duarte, S, Atkinson, D, Soneji, S, Domanski, A, Farley, A, Sanjuan-Pla, A, Carella, C, Patient, R, de Bruijn, M, Enver, T, Nerlov, C, Blackburn, C, Godin, I & Jacobsen, SEW 2012, 'The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.', Nature Immunology, vol. 13, no. 4, pp. 412-419. https://doi.org/10.1038/ni.2255

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T1 - The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.

AU - Luc, Sidinh

AU - Luis, Tiago C

AU - Boukarabila, Hanane

AU - Macaulay, Iain C

AU - Buza-Vidas, Natalija

AU - Bouriez-Jones, Tiphaine

AU - Lutteropp, Michael

AU - Woll, Petter S

AU - Loughran, Stephen J

AU - Mead, Adam J

AU - Hultquist, Anne

AU - Brown, John

AU - Mizukami, Takuo

AU - Matsuoka, Sahoko

AU - Ferry, Helen

AU - Anderson, Kristina

AU - Duarte, Sara

AU - Atkinson, Deborah

AU - Soneji, Shamit

AU - Domanski, Aniela

AU - Farley, Alison

AU - Sanjuan-Pla, Alejandra

AU - Carella, Cintia

AU - Patient, Roger

AU - de Bruijn, Marella

AU - Enver, Tariq

AU - Nerlov, Claus

AU - Blackburn, Clare

AU - Godin, Isabelle

AU - Jacobsen, Sten Eirik W

PY - 2012

Y1 - 2012

N2 - The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.

AB - The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.

U2 - 10.1038/ni.2255

DO - 10.1038/ni.2255

M3 - Article

C2 - 22344248

SN - 1529-2908

VL - 13

SP - 412

EP - 419

JO - Nature Immunology

JF - Nature Immunology

IS - 4

ER -

The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.

Abstract

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