Abstract
Phospholamban ablation in the mouse is associated with significant increases in cardiac contractility. To determine whether this hyperdynamic function persists through the aging process, a longitudinal examination of age-matched phospholamban-deficient and wild-type mice was employed. Kaplan-Meier survival curves indicated no significant differences between phospholamban-deficient and wild-type mice over the first year. Examination of cardiac function revealed significant increases in the rates of contraction (+dP/dt) and relaxation (-dP/dt) in phospholamban-deficient hearts compared with their wild-type counterparts at 3, 6, 12, 18 and 24 months of age. Quantitative immunoblotting indicated that the expression levels of the sarcoplasmic reticulum Ca2+-ATPase were not altered in wild-type hearts, while they were significantly decreased at 12 months (40%) and 18 months (20%) in phospholamban-deficient hearts. These findings on the persistence of hyperdynamic cardiac function over the long terra suggest that phospholamban may constitute an important target for treatment in heart disease.
Original language | English |
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Pages (from-to) | 1031-1040 |
Number of pages | 10 |
Journal | Journal of Molecular and Cellular Cardiology |
Volume | 33 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2001 |
Externally published | Yes |
Free keywords
- Aging
- Contractility
- Myocardium
- Phospholamban
- Sarcoplasmic reticulum