TY - JOUR
T1 - The GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization
AU - Lindström, Lisa
AU - Li, Tongbin
AU - Malycheva, Darina
AU - Kancharla, Arun
AU - Nilsson, Helén
AU - Vishnu, Neelanjan
AU - Mulder, Hindrik
AU - Johansson, Martin
AU - Rosselló, Catalina Ana
AU - Alvarado-Kristensson, Maria
PY - 2018/5/3
Y1 - 2018/5/3
N2 - In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with mitochondria. Our results demonstrate that γ-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that γ-tubulin provides a cytoskeletal element that gives form to the mitochondrial network.
AB - In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with mitochondria. Our results demonstrate that γ-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that γ-tubulin provides a cytoskeletal element that gives form to the mitochondrial network.
U2 - 10.1038/s42003-018-0037-3
DO - 10.1038/s42003-018-0037-3
M3 - Article
C2 - 30271923
SN - 2399-3642
VL - 1
JO - Communications Biology
JF - Communications Biology
IS - https://doi.org/10.1038/s42003-018-0037-3
M1 - 37
ER -