The immune cell atlas of human neuroblastoma

Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindström, David Gisselsson, Shahrzad Shirazi Fard, Catharina Hagerling, Peter V. Kharchenko, Per Kogner, John Inge Johnsen, Ninib Baryawno

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. Our receptor-ligand interaction analysis reveals a highly complex interactive network of the NB microenvironment from which we highlight several interactions that we suggest for future therapeutic studies.

Original languageEnglish
Article number100657
JournalCell Reports Medicine
Volume3
Issue number6
DOIs
Publication statusPublished - 2022 Jun 21

Subject classification (UKÄ)

  • Cell and Molecular Biology

Free keywords

  • cancer
  • human
  • immune cell landscape
  • immuno-oncology
  • immunotherapy
  • neuroblastoma
  • pediatric cancer
  • prognosis
  • single-cell RNA sequencing
  • survival

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