The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

Eskil Söderlind, Roland Carlsson, Carl Borrebaeck, Mats Ohlin

Research output: Contribution to journalArticlepeer-review

Abstract

Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.
Original languageEnglish
Pages (from-to)409-416
JournalCombinatorial Chemistry & High Throughput Screening
Volume4
Issue number5
Publication statusPublished - 2001

Subject classification (UKÄ)

  • Immunology in the medical area

Free keywords

  • COMPLEMENTARITY-DETERMINING REGIONS
  • PHAGE DISPLAY LIBRARY
  • SINGLE-CHAIN
  • FV
  • AFFINITY HUMAN-ANTIBODIES
  • BY-PASSING IMMUNIZATION
  • RANDOM
  • MUTAGENESIS
  • BINDING-SITE
  • V-H
  • SYNTHETIC REPERTOIRES
  • SOMATIC
  • HYPERMUTATION

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