The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.

Anna Andersson; Jing Ma; Jianmin Wang; Xiang Chen; Amanda Larson Gedman; Jinjun Dang; Joy Nakitandwe; Linda Holmfeldt; Matthew Parker; John Easton; Robert Huether; Richard Kriwacki; Michael Rusch; Gang Wu; Yongjin Li; Heather Mulder; Susana Raimondi; Stanley Pounds; Guolian Kang; Lei Shi; Jared Becksfort; Pankaj Gupta; Debbie Payne-Turner; Bhavin Vadodaria; Kristy Boggs; Donald Yergeau; Jayanthi Manne; Guangchun Song; Michael Edmonson; Panduka Nagahawatte; Lei Wei; Cheng Cheng; Deqing Pei; Rosemary Sutton; Nicola C Venn; Albert Chetcuti; Amanda Rush; Daniel Catchpoole; Jesper Heldrup; Thoas Fioretos; Charles Lu; Li Ding; Ching-Hon Pui; Sheila Shurtleff; Charles G Mullighan; Elaine R Mardis; Richard K Wilson; Tanja A Gruber; Jinghui Zhang; James R Downing

doi:10.1038/ng.3230

The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.

Anna Andersson, Jing Ma, Jianmin Wang, Xiang Chen, Amanda Larson Gedman, Jinjun Dang, Joy Nakitandwe, Linda Holmfeldt, Matthew Parker, John Easton, Robert Huether, Richard Kriwacki, Michael Rusch, Gang Wu, Yongjin Li, Heather Mulder, Susana Raimondi, Stanley Pounds, Guolian Kang, Lei ShiJared Becksfort, Pankaj Gupta, Debbie Payne-Turner, Bhavin Vadodaria, Kristy Boggs, Donald Yergeau, Jayanthi Manne, Guangchun Song, Michael Edmonson, Panduka Nagahawatte, Lei Wei, Cheng Cheng, Deqing Pei, Rosemary Sutton, Nicola C Venn, Albert Chetcuti, Amanda Rush, Daniel Catchpoole, Jesper Heldrup, Thoas Fioretos, Charles Lu, Li Ding, Ching-Hon Pui, Sheila Shurtleff, Charles G Mullighan, Elaine R Mardis, Richard K Wilson, Tanja A Gruber, Jinghui Zhang, James R Downing

Skåne University Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Infant acute lymphoblastic leukemia (ALL) with MLL rearrangements (MLL-R) represents a distinct leukemia with a poor prognosis. To define its mutational landscape, we performed whole-genome, exome, RNA and targeted DNA sequencing on 65 infants (47 MLL-R and 18 non-MLL-R cases) and 20 older children (MLL-R cases) with leukemia. Our data show that infant MLL-R ALL has one of the lowest frequencies of somatic mutations of any sequenced cancer, with the predominant leukemic clone carrying a mean of 1.3 non-silent mutations. Despite this paucity of mutations, we detected activating mutations in kinase-PI3K-RAS signaling pathway components in 47% of cases. Surprisingly, these mutations were often subclonal and were frequently lost at relapse. In contrast to infant cases, MLL-R leukemia in older children had more somatic mutations (mean of 6.5 mutations/case versus 1.3 mutations/case, P = 7.15 × 10(-5)) and had frequent mutations (45%) in epigenetic regulators, a category of genes that, with the exception of MLL, was rarely mutated in infant MLL-R ALL.

Original language	English
Pages (from-to)	330-U192
Journal	Nature Genetics
Volume	47
Issue number	4
DOIs	https://doi.org/10.1038/ng.3230
Publication status	Published - 2015

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SDG 3 Good Health and Well-being

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Medical Genetics and Genomics (including Gene Therapy)

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10.1038/ng.3230

http://www.ncbi.nlm.nih.gov/pubmed/25730765?dopt=Abstract

Cite this

Andersson, A., Ma, J., Wang, J., Chen, X., Gedman, A. L., Dang, J., Nakitandwe, J., Holmfeldt, L., Parker, M., Easton, J., Huether, R., Kriwacki, R., Rusch, M., Wu, G., Li, Y., Mulder, H., Raimondi, S., Pounds, S., Kang, G., ... Downing, J. R. (2015). The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias. Nature Genetics, 47(4), 330-U192. https://doi.org/10.1038/ng.3230

@article{f7c9026b837f4cd9a96179c808db376f,

title = "The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.",

abstract = "Infant acute lymphoblastic leukemia (ALL) with MLL rearrangements (MLL-R) represents a distinct leukemia with a poor prognosis. To define its mutational landscape, we performed whole-genome, exome, RNA and targeted DNA sequencing on 65 infants (47 MLL-R and 18 non-MLL-R cases) and 20 older children (MLL-R cases) with leukemia. Our data show that infant MLL-R ALL has one of the lowest frequencies of somatic mutations of any sequenced cancer, with the predominant leukemic clone carrying a mean of 1.3 non-silent mutations. Despite this paucity of mutations, we detected activating mutations in kinase-PI3K-RAS signaling pathway components in 47\% of cases. Surprisingly, these mutations were often subclonal and were frequently lost at relapse. In contrast to infant cases, MLL-R leukemia in older children had more somatic mutations (mean of 6.5 mutations/case versus 1.3 mutations/case, P = 7.15 × 10(-5)) and had frequent mutations (45\%) in epigenetic regulators, a category of genes that, with the exception of MLL, was rarely mutated in infant MLL-R ALL.",

author = "Anna Andersson and Jing Ma and Jianmin Wang and Xiang Chen and Gedman, \{Amanda Larson\} and Jinjun Dang and Joy Nakitandwe and Linda Holmfeldt and Matthew Parker and John Easton and Robert Huether and Richard Kriwacki and Michael Rusch and Gang Wu and Yongjin Li and Heather Mulder and Susana Raimondi and Stanley Pounds and Guolian Kang and Lei Shi and Jared Becksfort and Pankaj Gupta and Debbie Payne-Turner and Bhavin Vadodaria and Kristy Boggs and Donald Yergeau and Jayanthi Manne and Guangchun Song and Michael Edmonson and Panduka Nagahawatte and Lei Wei and Cheng Cheng and Deqing Pei and Rosemary Sutton and Venn, \{Nicola C\} and Albert Chetcuti and Amanda Rush and Daniel Catchpoole and Jesper Heldrup and Thoas Fioretos and Charles Lu and Li Ding and Ching-Hon Pui and Sheila Shurtleff and Mullighan, \{Charles G\} and Mardis, \{Elaine R\} and Wilson, \{Richard K\} and Gruber, \{Tanja A\} and Jinghui Zhang and Downing, \{James R\}",

year = "2015",

doi = "10.1038/ng.3230",

language = "English",

volume = "47",

pages = "330--U192",

journal = "Nature Genetics",

issn = "1546-1718",

publisher = "Nature Publishing Group",

number = "4",

}

Andersson, A, Ma, J, Wang, J, Chen, X, Gedman, AL, Dang, J, Nakitandwe, J, Holmfeldt, L, Parker, M, Easton, J, Huether, R, Kriwacki, R, Rusch, M, Wu, G, Li, Y, Mulder, H, Raimondi, S, Pounds, S, Kang, G, Shi, L, Becksfort, J, Gupta, P, Payne-Turner, D, Vadodaria, B, Boggs, K, Yergeau, D, Manne, J, Song, G, Edmonson, M, Nagahawatte, P, Wei, L, Cheng, C, Pei, D, Sutton, R, Venn, NC, Chetcuti, A, Rush, A, Catchpoole, D, Heldrup, J, Fioretos, T, Lu, C, Ding, L, Pui, C-H, Shurtleff, S, Mullighan, CG, Mardis, ER, Wilson, RK, Gruber, TA, Zhang, J & Downing, JR 2015, 'The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.', Nature Genetics, vol. 47, no. 4, pp. 330-U192. https://doi.org/10.1038/ng.3230

TY - JOUR

T1 - The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.

AU - Andersson, Anna

AU - Ma, Jing

AU - Wang, Jianmin

AU - Chen, Xiang

AU - Gedman, Amanda Larson

AU - Dang, Jinjun

AU - Nakitandwe, Joy

AU - Holmfeldt, Linda

AU - Parker, Matthew

AU - Easton, John

AU - Huether, Robert

AU - Kriwacki, Richard

AU - Rusch, Michael

AU - Wu, Gang

AU - Li, Yongjin

AU - Mulder, Heather

AU - Raimondi, Susana

AU - Pounds, Stanley

AU - Kang, Guolian

AU - Shi, Lei

AU - Becksfort, Jared

AU - Gupta, Pankaj

AU - Payne-Turner, Debbie

AU - Vadodaria, Bhavin

AU - Boggs, Kristy

AU - Yergeau, Donald

AU - Manne, Jayanthi

AU - Song, Guangchun

AU - Edmonson, Michael

AU - Nagahawatte, Panduka

AU - Wei, Lei

AU - Cheng, Cheng

AU - Pei, Deqing

AU - Sutton, Rosemary

AU - Venn, Nicola C

AU - Chetcuti, Albert

AU - Rush, Amanda

AU - Catchpoole, Daniel

AU - Heldrup, Jesper

AU - Fioretos, Thoas

AU - Lu, Charles

AU - Ding, Li

AU - Pui, Ching-Hon

AU - Shurtleff, Sheila

AU - Mullighan, Charles G

AU - Mardis, Elaine R

AU - Wilson, Richard K

AU - Gruber, Tanja A

AU - Zhang, Jinghui

AU - Downing, James R

PY - 2015

Y1 - 2015

N2 - Infant acute lymphoblastic leukemia (ALL) with MLL rearrangements (MLL-R) represents a distinct leukemia with a poor prognosis. To define its mutational landscape, we performed whole-genome, exome, RNA and targeted DNA sequencing on 65 infants (47 MLL-R and 18 non-MLL-R cases) and 20 older children (MLL-R cases) with leukemia. Our data show that infant MLL-R ALL has one of the lowest frequencies of somatic mutations of any sequenced cancer, with the predominant leukemic clone carrying a mean of 1.3 non-silent mutations. Despite this paucity of mutations, we detected activating mutations in kinase-PI3K-RAS signaling pathway components in 47% of cases. Surprisingly, these mutations were often subclonal and were frequently lost at relapse. In contrast to infant cases, MLL-R leukemia in older children had more somatic mutations (mean of 6.5 mutations/case versus 1.3 mutations/case, P = 7.15 × 10(-5)) and had frequent mutations (45%) in epigenetic regulators, a category of genes that, with the exception of MLL, was rarely mutated in infant MLL-R ALL.

AB - Infant acute lymphoblastic leukemia (ALL) with MLL rearrangements (MLL-R) represents a distinct leukemia with a poor prognosis. To define its mutational landscape, we performed whole-genome, exome, RNA and targeted DNA sequencing on 65 infants (47 MLL-R and 18 non-MLL-R cases) and 20 older children (MLL-R cases) with leukemia. Our data show that infant MLL-R ALL has one of the lowest frequencies of somatic mutations of any sequenced cancer, with the predominant leukemic clone carrying a mean of 1.3 non-silent mutations. Despite this paucity of mutations, we detected activating mutations in kinase-PI3K-RAS signaling pathway components in 47% of cases. Surprisingly, these mutations were often subclonal and were frequently lost at relapse. In contrast to infant cases, MLL-R leukemia in older children had more somatic mutations (mean of 6.5 mutations/case versus 1.3 mutations/case, P = 7.15 × 10(-5)) and had frequent mutations (45%) in epigenetic regulators, a category of genes that, with the exception of MLL, was rarely mutated in infant MLL-R ALL.

UR - https://www.scopus.com/pages/publications/84925841593

U2 - 10.1038/ng.3230

DO - 10.1038/ng.3230

M3 - Article

C2 - 25730765

SN - 1546-1718

VL - 47

SP - 330-U192

JO - Nature Genetics

JF - Nature Genetics

IS - 4

ER -

The landscape of somatic mutations in infant MLL-rearranged acute lymphoblastic leukemias.

Abstract

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