The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells

Göran Karlsson, Emma Rörby, Cristina Pina, Shamit Soneji, Kristian Reckzeh, Kenichi Miharada, Christine Karlsson, Yanping Guo, Cristina Fugazza, Rajeev Gupta, Joost H. A. Martens, Hendrik G. Stunnenberg, Stefan Karlsson, Tariq Enver

Research output: Contribution to journalArticlepeer-review

339 Downloads (Pure)

Abstract

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.
Original languageEnglish
Pages (from-to)642-648
JournalCell Reports
Volume4
Issue number4
DOIs
Publication statusPublished - 2013

Subject classification (UKÄ)

  • Cell Biology

Fingerprint

Dive into the research topics of 'The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells'. Together they form a unique fingerprint.

Cite this