The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor

Jan Tkac, James W. Whittaker, Tautgirdas Ruzgas

Research output: Contribution to journalArticlepeer-review

Abstract

Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 mu M) and very high assay throughput rate (150 h(-1)) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%.
Original languageEnglish
Pages (from-to)1820-1824
JournalBiosensors & Bioelectronics
Volume22
Issue number8
DOIs
Publication statusPublished - 2007

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry

Free keywords

  • galactose oxidase
  • chitosan
  • carbon nanotube
  • nation
  • interferents
  • blood plasma

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