Thermoregulatory costs of the innate immune response are modulated by winter food availability in a small passerine

In winter, a challenge to the immune system could pose a major energetic trade-off for small endotherms, whereby increasing body temperature (T_b; i.e. eliciting fever) may be beneficial to fight off invading pathogens yet incur a cost for vital energy-saving mechanisms. Having previously shown that the availability and acquisition of energy, through manipulation of food predictability, influences the depth of rest-phase hypothermia in a wild bird in winter, we expected that the nocturnal thermoregulatory component of the acute-phase immune response would also be modulated by food availability. By manipulating winter food availability in the wild for great tits Parus major, we created an area offering a “predictable” and constant supply of food at feeding stations, while an unmanipulated area was subject to naturally “unpredictable” food. Birds were subject to an immune challenge shortly after dusk, and the thermoregulatory response was quantified via continuous recording of nocturnal T_b, using subcutaneous thermo-sensitive transponders. In response to immune challenge, all birds increased T_b above the level maintained prior to immune challenge (i.e. baseline). However, birds experiencing a naturally unpredictable food supply elevated T_b more than birds subject to predictable food resources, during the period of expected peak response and for the duration of the night. Furthermore, “unpredictable-food” females took longer to return to their baseline T_b. Assuming baseline nocturnal T_b reflects an individual's optimum, based on their available energy budget, the metabolic cost of eliciting an acute-phase response for “unpredictable-food” birds was more than double that of “predictable-food” birds. The absence of differences in absolute T_b during the peak response could support the idea of an optimal T_b for immune system activation. Alternatively, “predictable-food” birds could have acquired tolerance to endotoxin as a result of using feeding stations, thus affording them reduced costs associated with a smaller T_b increase. These findings shed new light on the trade-offs associated with food acquisition, thermoregulation and immune function in small-bodied endotherms. This knowledge is of increasing importance, given the predicted elevated pathogen risks associated with changes in climate and anthropogenic activities.