Tissue microarray is inappropriate for analysis of BCL6 expression in diffuse large B-cell lymphoma.

Johan Linderoth, Mats Ehinger, Måns Åkerman, Eva Cavallin-Ståhl, Gunilla Enblad, Martin Erlanson, Mats Jerkeman

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Objective: In this study, our aim was to investigate how different immunohistochemical techniques may influence the result of BCL6 positivity and categorization in germinal center (GC) and non-GC derived diffuse large B-cell lymphoma (DLBCL), as it has been proposed that classification of DLBCL according to cell-of-origin by immunohistochemistry may be performed as a routine procedure in the diagnostic workup. However, a number of technical issues need to be solved before introducing this as a standard technique. Methods: Tumor specimens from 122 patients with de novo stage II-IV disease, adequately treated with anthracycline-containing chemotherapy regimens were collected. Immunohistochemical expression of BCL6, CD10, and MUM-1/IRF4 was examined using a tissue microarray (TMA) technique. BCL6 and CD10 were also evaluated on whole tissue sections. Results: Due to profound tissue heterogeneity, BCL6 showed a wide range of positivity, with a high number of false negative results by TMA (25% positive), compared to 53% on whole tissue sections (WTS). CD10 was more homogeneously expressed, and TMA results corresponded better to WTS. Consequently, the results from categorization into GC and non-GC DLBCL differed considerably by use of the two methods, and resulted in very different outcome in terms of overall survival. Conclusion: Immunohistochemical GC-status determined on TMA is not reliable enough to be used for individual treatment decisions in DLBCL, mostly due to difficulties in interpreting BCL6 status.
Original languageEnglish
Pages (from-to)146-149
JournalEuropean Journal of Haematology
Issue number2
Publication statusPublished - 2007

Subject classification (UKÄ)

  • Hematology


  • bCL6
  • microarray
  • tissue
  • diffuse large B-cell lymphoma
  • immunohistochemistry


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