Tissue microarray technique in soft tissue sarcoma: immunohistochemical Ki-67 expression in malignant fibrous histiocytoma

Jacob Engellau, Måns Åkerman, Harald Anderson, Henryk Domanski, Eva Rambech, Thor Alvegård, Mef Nilbert

Research output: Contribution to journalArticlepeer-review


Malignant fibrous histiocytoma (MFH) represents a heterogeneous soft tissue sarcoma entity. The authors compared different methods to determine immunohistochemical staining in whole tissue sections, evaluated the tissue microarray technique, and assessed immunohistochemical heterogeneity using the proliferation marker Ki-67 in 47 histopathologic tumor blocks from 11 MFHs. Whole tissue sections were assessed counting 400 cells along a line and counting all cells in 10 high-power fields (0.16 mm2) with mean Ki-67 expression levels of 13% and 11%, respectively. For the tissue microarray technique, two to three 0.6-mm diameter biopsies were studied from each of the 47 tumor blocks. Good correlation was obtained between whole tissue immunohistochemistry and tissue microarray with the microarray method, giving on average 8.6% greater Ki-67 expression levels than the reference method. Immunohistochemical tumor heterogeneity, evaluated using the high-power field method, showed a median standard deviation of 2.3% within the tumor blocks and 2.5% between the blocks from the same tumor. The authors concluded that the tissue microarray technique yields good quality staining and expression levels for Ki-67 comparable with whole tissue methods in MFH, but because of tumor heterogeneity, several tumor blocks ideally should be studied and, because of loss of material in the microarray process, multiple biopsies should be taken. The feasibility of tissue microarray for immunohistochemical studies of soft tissue sarcomas offers new possibilities to study multiple markers in large tumor materials.
Original languageEnglish
Pages (from-to)358-363
JournalApplied Immunohistochemistry & Molecular Morphology
Issue number4
Publication statusPublished - 2001

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Cancer Epidemiology (013007100), Oncology, MV (013035000), Reproductive Epidemiology/Tornblad Institute (013003000), Pathology, (Lund) (013030000)

Subject classification (UKÄ)

  • Cancer and Oncology


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