Abstract
This work focus on the functions by which the transcription factor Early B-cell Factor (EBF) regulate gene expression in different cell types. To address this question, gene expression analyses aimed to investigate the regulatory role of EBF in neuroblastoma cells, B-lymphocytes, adipocytes and stromal cells were performed.
The addresses question is of crucial importantse for the understanding of basic concepts in the area of developmental biology, were a limited set of transcription factors are able to support the development of different cell types. This could be achieved by that different differentiation-pathways share vital target genes involved in differentiation in general, or that TFs act on different target genes in different tissues. This work strongly suggests that in the case of EBF, it is an issue of regulation of different target genes in different tissues. The work indicates that the mechanism by which this is achieved would be a combination of coordinated expression of co-factors and chromatin configuration.
The addresses question is of crucial importantse for the understanding of basic concepts in the area of developmental biology, were a limited set of transcription factors are able to support the development of different cell types. This could be achieved by that different differentiation-pathways share vital target genes involved in differentiation in general, or that TFs act on different target genes in different tissues. This work strongly suggests that in the case of EBF, it is an issue of regulation of different target genes in different tissues. The work indicates that the mechanism by which this is achieved would be a combination of coordinated expression of co-factors and chromatin configuration.
Original language | English |
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Qualification | Doctor |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 2006 Oct 2 |
Publisher | |
ISBN (Print) | 91-85559-25-3 |
Publication status | Published - 2006 |
Bibliographical note
Defence detailsDate: 2006-10-02
Time: 13:00
Place: Segerfalk lecture hall Wallenberg Neurosciense Center, Sölvegatan 19, Lund
External reviewer(s)
Name: Rothenberg, Ellen
Title: Professor
Affiliation: California Institute of Technology, Pasadena, CA
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<div class="article_info">Anna Lagergren, Christina Manetopoulos, Håkan Axelson and Mikael Sigvardsson. <span class="article_issue_date">2004</span>. <span class="article_title">Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression.</span> <span class="journal_series_title">BMC Cancer</span>, <span class="journal_volume">vol 4</span> </div>
<div class="article_info">Robert Månsson, Panagiotis Tsapogas, Mikael Åkerlund, Anna Lagergren, Ramiro Gisler and Mikael Sigvardsson. <span class="article_issue_date">2004</span>. <span class="article_title">Pearson correlation analysis of microarray data allows for the identification of genetic targets for Early B-cell Factor.</span> <span class="journal_series_title">Journal of Biological Chemistry</span>, <span class="journal_volume">vol 279</span> <span class="journal_pages">pp 17905-17913</span>.</div>
<div class="article_info">Anna Lagergren, Robert Månsson, Emma Smith, Barbro Basta, David Bryder, Peter Åkerblad and Mikael Sigvardsson. <span class="article_issue_date"></span>. <span class="article_title">Early B-cell Factor regulates the expression of B-lineage growth factors in stromacells.</span> (submitted)</div>
<div class="article_info">Peter Åkerblad, Robert Månsson, Anna Lagergren, Simonetta Westerlund, Barbro Basta, Ulrika Lind, Anders Thelin, Ramiro Gisler, David Liberg, Sven Nelander, Krister Bamberg and Mikael Sigvardsson. <span class="article_issue_date">2005</span>. <span class="article_title">Gene expression analysis suggests that EBF-1 and PPAR?2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics.</span> <span class="journal_series_title">Physiological Genomics</span>, <span class="journal_volume">vol 23</span> <span class="journal_pages">pp 206-216</span>.</div>
<div class="article_info">Anna Lagergren, Robert Månsson, Frida Hansson, Simonetta Westerlund, Krister Bamberg, Evan Rosen, Mikael Sigvardsson and Peter Åkerblad. <span class="article_issue_date"></span>. <span class="article_title">EBF (O/E)-1 is a positive regulator of the IGFII and Glut-4 genes and stimulates adipogenic conversion of NIH3T3 cells with a reduced insulin dependency.</span> (manuscript)</div>
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012)
Subject classification (UKÄ)
- Cell and Molecular Biology
Free keywords
- transcription factor
- extracellular fluids
- Haematology
- tissue specific
- cellular differentiation
- EBF
- Hematologi
- extracellulära vätskor