Total internal reflection fluorescence microscopy to study GLUT4 trafficking

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6 Citations (SciVal)

Abstract

Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages151-159
Number of pages9
Volume1713
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1713
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Cell and Molecular Biology
  • Medical Image Processing

Keywords

  • Fluorescence
  • GLUT4
  • GLUT4 storage vesicles
  • GSV
  • Microscopy
  • Primary adipocytes
  • TIRF

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