Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves

Anna Fornell; Carl Johannesson; Sean S. Searle; Axel Happstadius; Johan Nilsson; Maria Tenje

doi:10.1109/TRANSDUCERS.2019.8808393

Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves

Anna Fornell, Carl Johannesson, Sean S. Searle, Axel Happstadius, Johan Nilsson, Maria Tenje

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

In this paper an acoustofluidic system to trap hydrogel droplets is shown. The presented trapping method is label-free, biocompatible and operated in non-contact mode. The results show that the droplets can be trapped at flow rates up to 76 μL/min which corresponds to an average flow speed of 3.2 mm/s. Moreover, it is shown that the droplets can be trapped for several hours, thus allowing for studies of the encapsulated cells over time. An application of the system is shown by performing on-chip cell nuclei staining.

Original language	English
Title of host publication	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Publisher	IEEE - Institute of Electrical and Electronics Engineers Inc.
Pages	2352-2355
Number of pages	4
ISBN (Electronic)	9781728120072
DOIs	https://doi.org/10.1109/TRANSDUCERS.2019.8808393
Publication status	Published - 2019
Event	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany Duration: 2019 Jun 23 → 2019 Jun 27

Conference

Conference	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Country/Territory	Germany
City	Berlin
Period	2019/06/23 → 2019/06/27

Subject classification (UKÄ)

Medical Laboratory Technologies

Free keywords

Acoustofluidics
droplet microfluidics
hydrogels
particle manipulation

Access to Document

10.1109/TRANSDUCERS.2019.8808393

Cite this

Fornell, A., Johannesson, C., Searle, S. S., Happstadius, A., Nilsson, J., & Tenje, M. (2019). Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 2352-2355). Article 8808393 IEEE - Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808393

Fornell, Anna ; Johannesson, Carl ; Searle, Sean S. et al. / Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. IEEE - Institute of Electrical and Electronics Engineers Inc., 2019. pp. 2352-2355

@inproceedings{b195cf1f5d6d40bc989bc389e55cf777,

title = "Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves",

abstract = "In this paper an acoustofluidic system to trap hydrogel droplets is shown. The presented trapping method is label-free, biocompatible and operated in non-contact mode. The results show that the droplets can be trapped at flow rates up to 76 μL/min which corresponds to an average flow speed of 3.2 mm/s. Moreover, it is shown that the droplets can be trapped for several hours, thus allowing for studies of the encapsulated cells over time. An application of the system is shown by performing on-chip cell nuclei staining.",

keywords = "Acoustofluidics, droplet microfluidics, hydrogels, particle manipulation",

author = "Anna Fornell and Carl Johannesson and Searle, {Sean S.} and Axel Happstadius and Johan Nilsson and Maria Tenje",

year = "2019",

doi = "10.1109/TRANSDUCERS.2019.8808393",

language = "English",

pages = "2352--2355",

booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",

publisher = "IEEE - Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII ; Conference date: 23-06-2019 Through 27-06-2019",

}

Fornell, A, Johannesson, C, Searle, SS, Happstadius, A, Nilsson, J & Tenje, M 2019, Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808393, IEEE - Institute of Electrical and Electronics Engineers Inc., pp. 2352-2355, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 2019/06/23. https://doi.org/10.1109/TRANSDUCERS.2019.8808393

Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves. / Fornell, Anna; Johannesson, Carl; Searle, Sean S. et al.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. IEEE - Institute of Electrical and Electronics Engineers Inc., 2019. p. 2352-2355 8808393.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves

AU - Fornell, Anna

AU - Johannesson, Carl

AU - Searle, Sean S.

AU - Happstadius, Axel

AU - Nilsson, Johan

AU - Tenje, Maria

PY - 2019

Y1 - 2019

N2 - In this paper an acoustofluidic system to trap hydrogel droplets is shown. The presented trapping method is label-free, biocompatible and operated in non-contact mode. The results show that the droplets can be trapped at flow rates up to 76 μL/min which corresponds to an average flow speed of 3.2 mm/s. Moreover, it is shown that the droplets can be trapped for several hours, thus allowing for studies of the encapsulated cells over time. An application of the system is shown by performing on-chip cell nuclei staining.

AB - In this paper an acoustofluidic system to trap hydrogel droplets is shown. The presented trapping method is label-free, biocompatible and operated in non-contact mode. The results show that the droplets can be trapped at flow rates up to 76 μL/min which corresponds to an average flow speed of 3.2 mm/s. Moreover, it is shown that the droplets can be trapped for several hours, thus allowing for studies of the encapsulated cells over time. An application of the system is shown by performing on-chip cell nuclei staining.

KW - Acoustofluidics

KW - droplet microfluidics

KW - hydrogels

KW - particle manipulation

U2 - 10.1109/TRANSDUCERS.2019.8808393

DO - 10.1109/TRANSDUCERS.2019.8808393

M3 - Paper in conference proceeding

SP - 2352

EP - 2355

BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

PB - IEEE - Institute of Electrical and Electronics Engineers Inc.

T2 - 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Y2 - 23 June 2019 through 27 June 2019

ER -

Fornell A, Johannesson C, Searle SS, Happstadius A, Nilsson J, Tenje M. Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. IEEE - Institute of Electrical and Electronics Engineers Inc. 2019. p. 2352-2355. 8808393 doi: 10.1109/TRANSDUCERS.2019.8808393

Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves

Abstract

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Subject classification (UKÄ)

Free keywords

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Cite this