Two-photon excitation in fluorescence polarization receptor-ligand binding assay

M E Tirri; R J Huttunen; J Toivonen; Pirkko Härkönen; J T Soini; P E Hanninen

doi:10.1177/1087057104273334

Two-photon excitation in fluorescence polarization receptor-ligand binding assay

M E Tirri, R J Huttunen, J Toivonen, Pirkko Härkönen, J T Soini, P E Hanninen

Department of Translational Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

Original language	English
Pages (from-to)	314-319
Journal	Journal of Biomolecular Screening
Volume	10
Issue number	4
DOIs	https://doi.org/10.1177/1087057104273334
Publication status	Published - 2005

Bibliographical note

Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:32.

Subject classification (UKÄ)

Cancer and Oncology

Free keywords

two-photon excitation
fluorescence polarization
high-throughput screening
estrogen receptor
drug discovery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1087057104273334

Cite this

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abstract = "Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.",

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author = "Tirri, {M E} and Huttunen, {R J} and J Toivonen and Pirkko H{\"a}rk{\"o}nen and Soini, {J T} and Hanninen, {P E}",

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T1 - Two-photon excitation in fluorescence polarization receptor-ligand binding assay

AU - Tirri, M E

AU - Huttunen, R J

AU - Toivonen, J

AU - Härkönen, Pirkko

AU - Soini, J T

AU - Hanninen, P E

N1 - Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:32.

PY - 2005

Y1 - 2005

N2 - Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

AB - Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

KW - two-photon excitation

KW - fluorescence polarization

KW - high-throughput screening

KW - estrogen receptor

KW - drug discovery

U2 - 10.1177/1087057104273334

DO - 10.1177/1087057104273334

M3 - Article

SN - 1087-0571

VL - 10

SP - 314

EP - 319

JO - Journal of Biomolecular Screening

JF - Journal of Biomolecular Screening

IS - 4

ER -

Two-photon excitation in fluorescence polarization receptor-ligand binding assay

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

UN SDGs

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