Abstract
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.
| Original language | English |
|---|---|
| Pages (from-to) | 314-319 |
| Journal | Journal of Biomolecular Screening |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 |
Bibliographical note
Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:32.UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Subject classification (UKÄ)
- Cancer and Oncology
Free keywords
- two-photon excitation
- fluorescence polarization
- high-throughput screening
- estrogen receptor
- drug discovery
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