Ubiquitous Structural Signaling in Bacterial Phytochromes.

Alexander Björling; Oskar Berntsson; Heikki Takala; Kevin D Gallagher; Hardik Patel; Emil Gustavsson; Rachael St Peter; Phu Duong; Angela Nugent; Fan Zhang; Peter Berntsen; Roberto Appio; Ivan Rajkovic; Heli Lehtivuori; Matthijs R Panman; Maria Hoernke; Stephan Niebling; Rajiv Harimoorthy; Tilman Lamparter; Emina A Stojković; Janne A Ihalainen; Sebastian Westenhoff

doi:10.1021/acs.jpclett.5b01629

Ubiquitous Structural Signaling in Bacterial Phytochromes.

Alexander Björling, Oskar Berntsson, Heikki Takala, Kevin D Gallagher, Hardik Patel, Emil Gustavsson, Rachael St Peter, Phu Duong, Angela Nugent, Fan Zhang, Peter Berntsen, Roberto Appio, Ivan Rajkovic, Heli Lehtivuori, Matthijs R Panman, Maria Hoernke, Stephan Niebling, Rajiv Harimoorthy, Tilman Lamparter, Emina A StojkovićJanne A Ihalainen, Sebastian Westenhoff

MAX IV Laboratory

Research output: Contribution to journal › Article › peer-review

Abstract

The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

Original language	English
Pages (from-to)	3379-3383
Journal	The Journal of Physical Chemistry Letters
Volume	6
Issue number	17
DOIs	https://doi.org/10.1021/acs.jpclett.5b01629
Publication status	Published - 2015

Subject classification (UKÄ)

Biochemistry and Molecular Biology

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Björling, A., Berntsson, O., Takala, H., Gallagher, K. D., Patel, H., Gustavsson, E., St Peter, R., Duong, P., Nugent, A., Zhang, F., Berntsen, P., Appio, R., Rajkovic, I., Lehtivuori, H., Panman, M. R., Hoernke, M., Niebling, S., Harimoorthy, R., Lamparter, T., ... Westenhoff, S. (2015). Ubiquitous Structural Signaling in Bacterial Phytochromes. The Journal of Physical Chemistry Letters, 6(17), 3379-3383. https://doi.org/10.1021/acs.jpclett.5b01629

@article{a14602b12bee48f78ae53461da664bd9,

title = "Ubiquitous Structural Signaling in Bacterial Phytochromes.",

abstract = "The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.",

author = "Alexander Bj{\"o}rling and Oskar Berntsson and Heikki Takala and Gallagher, {Kevin D} and Hardik Patel and Emil Gustavsson and {St Peter}, Rachael and Phu Duong and Angela Nugent and Fan Zhang and Peter Berntsen and Roberto Appio and Ivan Rajkovic and Heli Lehtivuori and Panman, {Matthijs R} and Maria Hoernke and Stephan Niebling and Rajiv Harimoorthy and Tilman Lamparter and Stojkovi{\'c}, {Emina A} and Ihalainen, {Janne A} and Sebastian Westenhoff",

year = "2015",

doi = "10.1021/acs.jpclett.5b01629",

language = "English",

volume = "6",

pages = "3379--3383",

journal = "The Journal of Physical Chemistry Letters",

issn = "1948-7185",

publisher = "The American Chemical Society (ACS)",

number = "17",

}

Björling, A, Berntsson, O, Takala, H, Gallagher, KD, Patel, H, Gustavsson, E, St Peter, R, Duong, P, Nugent, A, Zhang, F, Berntsen, P, Appio, R, Rajkovic, I, Lehtivuori, H, Panman, MR, Hoernke, M, Niebling, S, Harimoorthy, R, Lamparter, T, Stojković, EA, Ihalainen, JA & Westenhoff, S 2015, 'Ubiquitous Structural Signaling in Bacterial Phytochromes.', The Journal of Physical Chemistry Letters, vol. 6, no. 17, pp. 3379-3383. https://doi.org/10.1021/acs.jpclett.5b01629

TY - JOUR

T1 - Ubiquitous Structural Signaling in Bacterial Phytochromes.

AU - Björling, Alexander

AU - Berntsson, Oskar

AU - Takala, Heikki

AU - Gallagher, Kevin D

AU - Patel, Hardik

AU - Gustavsson, Emil

AU - St Peter, Rachael

AU - Duong, Phu

AU - Nugent, Angela

AU - Zhang, Fan

AU - Berntsen, Peter

AU - Appio, Roberto

AU - Rajkovic, Ivan

AU - Lehtivuori, Heli

AU - Panman, Matthijs R

AU - Hoernke, Maria

AU - Niebling, Stephan

AU - Harimoorthy, Rajiv

AU - Lamparter, Tilman

AU - Stojković, Emina A

AU - Ihalainen, Janne A

AU - Westenhoff, Sebastian

PY - 2015

Y1 - 2015

N2 - The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

AB - The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

U2 - 10.1021/acs.jpclett.5b01629

DO - 10.1021/acs.jpclett.5b01629

M3 - Article

C2 - 26275765

SN - 1948-7185

VL - 6

SP - 3379

EP - 3383

JO - The Journal of Physical Chemistry Letters

JF - The Journal of Physical Chemistry Letters

IS - 17

ER -

Ubiquitous Structural Signaling in Bacterial Phytochromes.

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