Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids

Yannick Nuapia Belo, Said Al-Hamimi, Luke Chimuka, Charlotta Turner

Research output: Contribution to journalArticlepeer-review

12 Citations (SciVal)

Abstract

An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO 2 was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO 2 was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP.

Original languageEnglish
Pages (from-to)3685-3693
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number16
Early online date2019
DOIs
Publication statusPublished - 2019

Subject classification (UKÄ)

  • Analytical Chemistry

Keywords

  • Lipid profiling
  • Moringa oleifera
  • Moringa peregrina
  • Phospholipids
  • SFE
  • Sonication
  • Ultrahigh-pressure supercritical fluid extraction

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