Ultrastructure of alpha-synuclein-positive aggregations in U373 astrocytoma and rat primary glial cells

N Stefanova, Mia Emgård-Mattson, L Klimaschewski, GK Wenning, M Reindl

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17 Citations (SciVal)

Abstract

Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is intensely alpha-synuclein-immunopositive and a predominantly filamentous halo around. Mainly filamentous structures at the border area between the halo and the core are alpha-synuclein-immunoreactive. We conclude that this in vitro model of alpha-synuclein-positive glial aggregations mimics the morphology of the abnormal glial inclusions described in neuroclegenerative disorders and could be a suitable model for studying their role in the pathogenesis of these diseases. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)37-40
JournalNeuroscience Letters
Volume323
Issue number1
DOIs
Publication statusPublished - 2002

Subject classification (UKÄ)

  • Neurosciences

Keywords

  • glial cytoplasmic inclusions
  • ultrastructure
  • alpha-synuclein
  • glia
  • Lewy bodies
  • neurodegeneration

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