TY - JOUR
T1 - Ultrastructure of the epiphyseal plate of the guinea pig in experimental scurvy
AU - Thyberg, J.
AU - Lohmander, Stefan
AU - Friberg, U.
PY - 1971/12
Y1 - 1971/12
N2 - The ultrastructure of the upper tibial epiphyseal plate during the development of acute, experimental scurvy in the guinea pig was investigated. The cartilage was examined after the animals had been maintained, under standardized conditions, on a scorbutogenic diet for 8, 15, or 23 days. Progressive fine structural changes were observed in the experimental animals. Within the chondrocytes, the cisternae of the endoplasmic reticulum were rounded, non-continuous, and reduced in extensiveness. There was a decreased number of ribosomes on the cisternal membranes and, concomitantly, an increased amount of free ribosomes in the ground cytoplasm. The secretory vacuoles of the Golgi complex were few. Lysosomes were more frequent than in the controls. Signs of autophagocytosis were often observed. In the ground cytoplasm lipid droplets occurred somewhat more often than in the control animals. In the intercellular substance the amount of collagen fibrils and proteoglycan particles was decreased. Electron-dense matrix vesicles were numerous in the longitudinal septa. The nature of these changes is discussed with regard to the biochemical alterations that have been observed in this disorder.
AB - The ultrastructure of the upper tibial epiphyseal plate during the development of acute, experimental scurvy in the guinea pig was investigated. The cartilage was examined after the animals had been maintained, under standardized conditions, on a scorbutogenic diet for 8, 15, or 23 days. Progressive fine structural changes were observed in the experimental animals. Within the chondrocytes, the cisternae of the endoplasmic reticulum were rounded, non-continuous, and reduced in extensiveness. There was a decreased number of ribosomes on the cisternal membranes and, concomitantly, an increased amount of free ribosomes in the ground cytoplasm. The secretory vacuoles of the Golgi complex were few. Lysosomes were more frequent than in the controls. Signs of autophagocytosis were often observed. In the ground cytoplasm lipid droplets occurred somewhat more often than in the control animals. In the intercellular substance the amount of collagen fibrils and proteoglycan particles was decreased. Electron-dense matrix vesicles were numerous in the longitudinal septa. The nature of these changes is discussed with regard to the biochemical alterations that have been observed in this disorder.
UR - http://www.scopus.com/inward/record.url?scp=0015004377&partnerID=8YFLogxK
U2 - 10.1007/BF02894034
DO - 10.1007/BF02894034
M3 - Article
C2 - 4329482
AN - SCOPUS:0015004377
SN - 0042-6431
VL - 9
SP - 45
EP - 57
JO - Virchows Archiv B Cell Pathology Zell-pathologie
JF - Virchows Archiv B Cell Pathology Zell-pathologie
IS - 1
ER -