Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

Seifollah Azadi; Francois Paquet-Durand; Patrik Medstrand; Theo van Veen; Per Ekström

doi:10.1016/j.mcn.2006.01.001

Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

Seifollah Azadi, Francois Paquet-Durand, Patrik Medstrand, Theo van Veen, Per Ekström

Research output: Contribution to journal › Article › peer-review

Abstract

The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.

Original language	English
Pages (from-to)	759-773
Journal	Molecular and Cellular Neuroscience
Volume	31
Issue number	4
DOIs	https://doi.org/10.1016/j.mcn.2006.01.001
Publication status	Published - 2006

Subject classification (UKÄ)

Basic Medicine
Other Clinical Medicine

Free keywords

Apoptosis
Kinase
Microarray
Photoreceptor
Signaling pathways

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10.1016/j.mcn.2006.01.001

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16503160&dopt=Abstract

Cite this

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title = "Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.",

abstract = "The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.",

keywords = "Apoptosis, Kinase, Microarray, Photoreceptor, Signaling pathways",

author = "Seifollah Azadi and Francois Paquet-Durand and Patrik Medstrand and {van Veen}, Theo and Per Ekstr{\"o}m",

year = "2006",

doi = "10.1016/j.mcn.2006.01.001",

language = "English",

volume = "31",

pages = "759--773",

journal = "Molecular and Cellular Neuroscience",

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T1 - Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

AU - Azadi, Seifollah

AU - Paquet-Durand, Francois

AU - Medstrand, Patrik

AU - van Veen, Theo

AU - Ekström, Per

PY - 2006

Y1 - 2006

N2 - The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.

AB - The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.

KW - Apoptosis

KW - Kinase

KW - Microarray

KW - Photoreceptor

KW - Signaling pathways

U2 - 10.1016/j.mcn.2006.01.001

DO - 10.1016/j.mcn.2006.01.001

M3 - Article

C2 - 16503160

SN - 1044-7431

VL - 31

SP - 759

EP - 773

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

IS - 4

ER -

Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

Abstract

Subject classification (UKÄ)

Free keywords

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