Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

Seifollah Azadi, Francois Paquet-Durand, Patrik Medstrand, Theo van Veen, Per Ekström

Research output: Contribution to journalArticlepeer-review

25 Citations (SciVal)

Abstract

The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.
Original languageEnglish
Pages (from-to)759-773
JournalMolecular and Cellular Neuroscience
Volume31
Issue number4
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Basic Medicine
  • Other Clinical Medicine

Keywords

  • Apoptosis
  • Kinase
  • Microarray
  • Photoreceptor
  • Signaling pathways

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