Use of microcalorimetry in analysing the kinetics of ADCC

J. Ankerst; H. O. Sjögren; R. Fäldt

doi:10.1016/0022-1759(86)90014-1

Use of microcalorimetry in analysing the kinetics of ADCC

J. Ankerst, H. O. Sjögren, R. Fäldt

Medicine/Emergency Medicine, Lund

Skåne University Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the G_D3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to G_D3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.

Original language	English
Pages (from-to)	259-264
Journal	Journal of Immunological Methods
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90014-1
Publication status	Published - 1986 Apr 17

Subject classification (UKÄ)

Cancer and Oncology

Free keywords

Antibody-dependent cellular cytotoxicity
Human lymphocytes
Microcalorimetry
Monoclonal antibody

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0022-1759(86)90014-1

Cite this

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title = "Use of microcalorimetry in analysing the kinetics of ADCC",

abstract = "Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.",

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author = "J. Ankerst and Sj{\"o}gren, {H. O.} and R. F{\"a}ldt",

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T1 - Use of microcalorimetry in analysing the kinetics of ADCC

AU - Ankerst, J.

AU - Sjögren, H. O.

AU - Fäldt, R.

PY - 1986/4/17

Y1 - 1986/4/17

N2 - Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.

AB - Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.

KW - Antibody-dependent cellular cytotoxicity

KW - Human lymphocytes

KW - Microcalorimetry

KW - Monoclonal antibody

U2 - 10.1016/0022-1759(86)90014-1

DO - 10.1016/0022-1759(86)90014-1

M3 - Article

C2 - 3958501

AN - SCOPUS:0022652227

SN - 0022-1759

VL - 88

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EP - 264

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Use of microcalorimetry in analysing the kinetics of ADCC

Abstract

Subject classification (UKÄ)

Free keywords

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