Abstract
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells.
| Original language | English |
|---|---|
| Title of host publication | Human Embryonic and Induced Pluripotent Stem Cells |
| Subtitle of host publication | Part of the series Springer Protocols Handbooks |
| Editors | Kaiming Ye, Sha Jin |
| Publisher | Springer |
| Pages | 465-480 |
| ISBN (Electronic) | 978-1-61779-267-0 |
| ISBN (Print) | 978-1-61779-266-3 |
| Publication status | Published - 2011 Jul 30 |
Subject classification (UKÄ)
- Neurosciences
Free keywords
- pluripotent stem cells
- microRNA