Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

E.J.C. Nilsson; Magnus Gram; J. Nilsson; Jan Pallon; Anna Masternak; J. Paczesny; Natalia Arteaga; Mikael Elfman; Per Kristiansson; Charlotta Nilsson; Bo Åkerström

doi:10.1016/j.nimb.2009.02.051

Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

E.J.C. Nilsson, Magnus Gram, J. Nilsson, Jan Pallon, Anna Masternak, J. Paczesny, Natalia Arteaga, Mikael Elfman, Per Kristiansson, Charlotta Nilsson, Bo Åkerström

Infection Medicine (BMC)

Research output: Contribution to journal › Article › peer-review

Abstract

In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	1199-1205
Journal	Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
Volume	267
Issue number	7
DOIs	https://doi.org/10.1016/j.nimb.2009.02.051
Publication status	Published - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)

Subject classification (UKÄ)

Subatomic Physics

Free keywords

irradiation
Microbeam
Patterned cell dish
Microdispensing
Cell substrate
Bystander effect
HepG2 cells

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10.1016/j.nimb.2009.02.051

Cite this

Nilsson, E. J. C., Gram, M., Nilsson, J., Pallon, J., Masternak, A., Paczesny, J., Arteaga, N., Elfman, M., Kristiansson, P., Nilsson, C., & Åkerström, B. (2009). Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells. Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, 267(7), 1199-1205. https://doi.org/10.1016/j.nimb.2009.02.051

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title = "Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells",

abstract = "In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.",

keywords = "irradiation, Microbeam, Patterned cell dish, Microdispensing, Cell substrate, Bystander effect, HepG2 cells",

author = "E.J.C. Nilsson and Magnus Gram and J. Nilsson and Jan Pallon and Anna Masternak and J. Paczesny and Natalia Arteaga and Mikael Elfman and Per Kristiansson and Charlotta Nilsson and Bo {\AA}kerstr{\"o}m",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)",

year = "2009",

doi = "10.1016/j.nimb.2009.02.051",

language = "English",

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Nilsson, EJC, Gram, M, Nilsson, J, Pallon, J, Masternak, A, Paczesny, J, Arteaga, N, Elfman, M , Kristiansson, P , Nilsson, C & Åkerström, B 2009, 'Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells', Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, vol. 267, no. 7, pp. 1199-1205. https://doi.org/10.1016/j.nimb.2009.02.051

Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells. / Nilsson, E.J.C.; Gram, Magnus; Nilsson, J. et al.
In: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, Vol. 267, No. 7, 2009, p. 1199-1205.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

AU - Nilsson, E.J.C.

AU - Gram, Magnus

AU - Nilsson, J.

AU - Pallon, Jan

AU - Masternak, Anna

AU - Paczesny, J.

AU - Arteaga, Natalia

AU - Elfman, Mikael

AU - Kristiansson, Per

AU - Nilsson, Charlotta

AU - Åkerström, Bo

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)

PY - 2009

Y1 - 2009

N2 - In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.

AB - In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.

KW - irradiation

KW - Microbeam

KW - Patterned cell dish

KW - Microdispensing

KW - Cell substrate

KW - Bystander effect

KW - HepG2 cells

U2 - 10.1016/j.nimb.2009.02.051

DO - 10.1016/j.nimb.2009.02.051

M3 - Article

SN - 0168-583X

VL - 267

SP - 1199

EP - 1205

JO - Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms

IS - 7

ER -

Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

Access to Document

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