Variations of wettability and protein adsorption on solid siliceous carriers grafted with poly(N-isopropylacrylamide)

Alexander Ivanov, J Ekeroth, Lars Nilsson, Bo Mattiasson, Björn Bergenståhl, Igor Galaev

Research output: Contribution to journalArticlepeer-review


Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C. Adsorption isotherms of myoglobin indicate completely different characters of the protein adsorption to silica gel and its polyNIPAM-grafted derivative. Cooling of the grafted carrier containing adsorbed myoglobin to 9 degrees C led to a partial release of the protein to the contacting solution, whereas heating of the system to 35 degrees C resulted in reversible binding of the protein. Adsorption of myoglobin on polyNIPAM-coated silica was ca. 2-fold higher at 35 than at 9 degrees C, most probably (c) 2005 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)538-544
JournalJournal of Colloid and Interface Science
Issue number2
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Food Engineering
  • Industrial Biotechnology


  • thermoresponsive polymers
  • silica
  • grafting
  • protein adsorption


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